The Inoue Method for Preparation and Transformation of ...
Chemically-competent Transformation. 1.- Gently thaw frozen competent bacterial cells on ice. Aliquot 50 ul chemically-competent E. coli cells (e.g. strain JM109 or DH5α) into an appropriate number of sterile, labeled 1.5 ml tubes on ice (keeping cells ice cold at all times … ................
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