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Supplementary Methods. A: Quantitative analysis of immune activation, senescence and inflammation markers. B cell activation was assessed by . levels of B-cell-activating factor (BAFF) (Quantikine ELISA DBLYS0B, R&D Systems).. Monocyte activation was assessed by levels of sCD163 (Quantikine ELISA DC1630, R&D Systems), sCD14 (Flex Set, Cytometric Bead Array (CBA)) 560418, Becton Dickinson) … ................
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