CONFOCAL LASER SCANNING MICROSCOPY TUTORIAL

40X 0.75 dry 0.4 µm 0.26 µm 1.7 µm 1.2 µm 40X 1.3 oil 0.23 µm 0.15 µm 0.87 µm 0.61 µm 63X 1.4 oil 0.21 µm 0.14 µm 0.75 µm 0.53 µm Table 1. These data are based on the above equations using a wavelength of 488 nm (blue light) and a refractive index of 1 for the dry lenses and 1.515 for the oil lenses. The Pinhole. ................
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