DILUTIONS AND STANDARDS
In the second tube 2 mL of the first dilution was added to 2 mL of saline: 1/4 x 2/4 or 1/4 x 1/2 = 1/8 in 2nd tube . By transferring 2 mL of dilution from one tube to a second tube containing 2 mL of saline, and continuing to do so, this is actually making a 1:2 serial dilution. PROBLEMS: 1. You made a 1:2 dilution of serum. ................
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