Kinetex EVO C18 - Phenomenex

Core-Shell Technology

Pssst! Waters? XBridge?

and BEHTM user,

now you can...

Triumph

with ? Improved Peak Shape for Bases ? Shorter Run Times ? Core-Shell Performance from pH 1-12

Kinetex EVO C18

Core-Shell UHPLC/HPLC Columns

EVO C18

The Past

Traditional fully porous hybrid

? More band broadening ? Lower sensitivities ? Longer run times

The Present

Kinetex? Core-Shell EVO C18

? Sharper peaks ? Higher efficiencies

? Increased sensitivity ? Shorter run times

More Band Broadening

Less Band Broadening

Introducing Kinetex EVO C18

The Kinetex core-shell EVO C18 provides much greater LC performance than fully porous products, giving you the freedom to get improved results, cost savings, and overall higher productivity. The patented surface technology of the Kinetex EVO C18 also provides you with the ruggedness to take on 100% aqueous conditions and reversed phase methods from pH 1-12.

O

O

O

O O

O CH2 O

SiSCi HC2 HC2H2SiSi

O

O

O O

O CH2

SiSCi HC2 HC2H2SiS

O

O

O

CH2

CH2

Si CH2Si

CH2 O

O O

O

O

CH2

SiSCi HC2 HC2HS2 iSi

O

O

O O

OC SCi H2

Si C O

O CH2 O

O O

SiSCi HC2 HC2HS2 iSi

O

O

O O

O Si

SCi

HC2HC2 HS2OiSCiHC2 CH2

O

O

O

O

Kinetex EVO C18 uses a patented organo-silica grafting process which incorporates uniform stabilizing ethane cross-linking to provide resistance to high pH attack while maintaining mechanical strength of the coreshell particle.

Kinetex EVO is patented by Phenomenex. U.S. Patent Nos. 7,563,367 and 8,658,038 and foreign counterparts.

? 2015 Phenomenex, Inc. All rights reserved.

2 l Phenomenex WEB:

Core-Shell Technology

How Does Kinetex EVO C18 Perform?

" With our vast range of clients, our quality control lab receives all kind of sample to analyse. This column is often used to analyse a large range of samples. The low cost combined with the great range of pH in which this column can be used saves us a lot of " trouble and gives us a great price/utility ratio. Pierre-Alexandre Paquette Solartech Laboratories, Inc.

" This column works better than any column " I have used for method development.

Jenn Jazikoff Dynamic Pharmaceuticals

Upgrade from XBridge and BEH C18 columns:

EVO C18

TM

XBridge? and BEHTM C18

3.5?m

vs

5?m

vs

1.7?m

vs

1.7?m

vs

5?m OBDTM

vs

Kinetex EVO C18

5?m 5?m 2.6?m 1.7?m 5?m AXIATM

Ordering Information

Page(s)

4 4-5 6-7 8-9 10

11

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3

Upgrade Your HPLC Methods for

Greater Sensitivity

With the combination of 100% aqueous stability, pH ruggedness from 1-12, and the core-shell performance advantage, you can easily replace old hybrid silica columns and gain immediate method improvements without increasing backpressure.

Kinetex? 5?m EVO

C18 22718 150 x 4.6mm

mAU

140

6

120

100

5

80

7

2

4

60

40 1

20

0

0

2

3 4

EVO C18

6

8

Conditions for all columns:

Column: Kinetex 5?m EVO C18 XBridge 5?m C18 XBridge 3.5?m C18

Dimensions: 150 x 4.6mm

Mobile Phase: A: 0.1% Formic acid in Water B: 0.1% Formic acid in Acetonitrile

Gradient: 5% to 95% B over 10 minutes

Flow Rate: 1.5mL/min

Temperature: Ambient

Detection: UV @ 254 nm

Sample: 1. Uracil 2. Pindolol 3. Chlorpheniramine 4. Nortriptyline 5. 3-Methyl-4-Nitrobenzoic acid 6. 5-Methyl Salicyl Aldehyde 7. Hexaphenone

143 Bar

Waters? XBridge? 3.5?m C18 22719 150 x 4.6mm

mAU 140

120

What an100increase in sensitivit8y0 & peak shape!

364 Bar

60

APP ID 22719

APP ID 22718

40

20 1

0

6 5

2 3

7 4

10

min

0

2

4

6

8

10

12

14 min

Waters XBridge 5?m C18 22720 150 x 4.6mm

mAU 140

120

100

80

60

40

20 1

0

0

2

6 5

2 3

4

6

8

171 Bar

APP ID 22720

7 4

10

12

14 min

Comparative separations may not be representative of all applications.

4 l Phenomenex WEB:

Advance pH 1-12 Methods Ahead with

Better Peak Shape

Core-Shell Technology

Easy improvements in resolution, productivity, and sensitivity are just a column away. By upgrading your methods from traditional fully porous hybrid materials to Kinetex 5?m EVO C18 Core-Shell Technology you can expect excellent performance and pH durability at low HPLC backpressures!

Kinetex 5?m EVO C18 150 22779 x 4.6mm

mAU

30

2 25

20

1

15

10

5

3 4

5

0

0

2

4

6

8

10

pH 7.0

APP ID 22779

12

14 min

Waters? XBridge? 5?m C18 150 22768 x 4.6mm

mAU

30

25

20 2

15 1

10

5

3

4

5

0

0

2

4

6

8

10

pH 7.0

APP ID 22788

12

14 min

mAU

14

2

mAU

pH 10.2 14

pH 10.2

APP ID 23108

12

3

10

4

8 1

6

5

4

12 2

10

3

8

4

1 6

5

4

APP ID 23107

2

2

0

0

0

2

4

6

8

10

12

14 min

0

2

4

6

8

10

12

14 min

Conditions for all columns:

Column: Kinetex 5?m EVO C18 XBridge 5?m C18

Dimensions: 150 x 4.6mm

Mobile Phase: A: 20 mM Sodium Phosphate (pH 7.0 or pH 10.2) B: Methanol/Acetonitrile (50:50)

Isocratic: A/B (30:70)

Flow Rate: 1.25mL/min

Temperature: 30 ?C

Detection: UV @ 254 nm

Sample: 1. Protriptyline 2. Nortriptyline 3. Imipramine 4. Amitriptyline 5. Clomipramine

Wow! Better peak shape and better performance in less time!

EVO C18

Comparative separations may not be representative of all applications.

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