Solutions and reagents for ELISA - Indoor Biotechnologies
• Solution A = 0.1M anhydrous citric acid, 19.21g/L • Solution = 0.2M Dibasic Na Phosphate.7H2O, 53.65g/L For 500ml buffer, mix 147ml A + 103ml B and make up to 500ml with deionized H2O. Add 274mg ABTS to 500ml buffer to make the substrate solution (contains 1mM ABTS). ABTS = 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid), Sigma A1888. ................
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