Specifications for High Density Polyethylene Geomembrane
50 mM Tris-HCl pH 8.0. 1 mM EDTA. 1 mM DTT. Protein Purification: Add His-protease inhibitor cocktail to thawed cells. Add MgCl2 (to ~10 mM from 1M stock) to the cells and a small scoop of solid DNase and RNase. Run cell suspension twice through the French Press while making sure to add 50 uL of 250 mM PMSF (43mg PMSF per 1 mL EtOH) after each ... ................
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