Methods Supplement - Nature

2. We used GM-CSF-conditioned media that contains 1-2ug GM-CSF/ml. We use this conditioned medium at a 1:100 dilution in our final medium, yielding a final GM-CSF concentration of 10-20ng/ml. B. Derivation procedure: 1. It is a good idea to keep a few wells of “uninfected” or “vector alone” infected cells maintained under the same ... ................
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