PcDNA™3.1(+) pcDNA™3.1(–) - Thermo Fisher Scientific
pcDNATM3.1(+) pcDNATM3.1(?)
Catalog nos. V790-20 and V795-20 Version K 10 November 2010 28-0104
User Manual
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Table of Contents
Important Information........................................................................................................................v Accessory Products ............................................................................................................................vi
Methods ......................................................................................................................... 1
Overview .............................................................................................................................................. 1 Cloning into pcDNATM3.1 .................................................................................................................. 2 Transfection .......................................................................................................................................... 6 Creating Stable Cell Lines .................................................................................................................. 7
Appendix...................................................................................................................... 10
pcDNATM3.1 Vectors ......................................................................................................................... 10 pcDNATM3.1/CAT............................................................................................................................. 12 Technical Support.............................................................................................................................. 13 Purchaser Notification ...................................................................................................................... 13 References ........................................................................................................................................... 15
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Important Information
pcDNATM Vectors
This manual is supplied with the following products.
Product pcDNATM3.1(+) Vector pcDNATM3.1(?) Vector
Catalog no. V790-20 V795-20
Shipping and Vectors are shipped on wet ice. Upon receipt, store at
Storage
?20?C.
Contents
The pcDNATM3.1 vector components pcDNATM3.1 are listed below:
Item
pcDNATM3.1 Vector pcDNATM3.1(+) or pcDNATM3.1(?)
Control Plasmid pcDNATM3.1/CAT
Concentration
20 g at 0.5 g/l, in TE buffer, pH 8.0 (10 mM Tris-HCl, 1 mM EDTA, pH 8.0)
Volume 40 l
20 g at 0.5 g/l, in TE buffer, pH 8.0 40 l (10 mM Tris-HCl, 1 mM EDTA, pH 8.0)
Product Qualification
The Certificate of Analysis provides detailed quality control information for each product. Certificates of Analysis are available on our website. Go to support and search for the Certificate of Analysis by product lot number, which is printed on the box.
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