Tissue Fixation and Freezing

2.) Place tissue in 30% sucrose for 24 hours having changed the sucrose 2-3x within 24 hours. Provide enough 30% sucrose to submerge the tissue. 3.) Place a plastic beaker containing 2-Methylbutane (Fisher Cat No. O3551-4) in an ice bucket, Styrofoam box or equivalent container containing a few inches of liquid nitrogen. ................
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