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mutation, sheared the DNA and made serial dilutions of DNA for each of them into wild-type DNA at 1:100, 1:300, 1:1,000, 1:3,000, and 1:10,000.1,2 After designing primers and probes for the various reactions, we tested the serial dilutions in the appropriate ddPCR reaction on the RainDance instrument (Supplemental eFigure S3), and constructed dilution series curves (Supplemental eFigure S4). ................
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