Determination of Biochemical Oxygen Demand (BOD5)

1. Reagent grade glucose. 2. Reagent grade glutamic acid. Procedure: 1. Dry reagent grade glucose and glutamic acid at 103(C for 1 hour and cool for one hour in the desiccator. 2. Dissolve 150 mg (0.150 g) of glucose and 150 mg (0.150 g) of glutamic acid in distilled water and bring up to 1 L. ................
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