Buffers and stocks - University of Pittsburgh
Ethidium Bromide Stock solution, 10 mg/ml. Ethidium Bromide 400 mg. Water 40 ml. Store at 4 ˚C. 5x Laemmli Sample buffer. SDS 1.5g. 1M Tris pH 6.8 3.75ml. Bromophenol blue 0.015g. DTT 1.16g. Glycerol 7.5ml. Mix thoroughly with stirring. Add water until 15ml total volume. Make 1ml aliquots and store at -20˚C. 1M MgCl2. MgCl2, 6H2O 10.17g ................
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