DropSense 96 and DropQuant v1 - PerkinElmer

[Pages:70]DropSense 96 and DropQuant v1.5

User manual June 2015

Contents

Contents

2

Trinean warranty statement

5

Introduction

6

Warning

6

Safety Information

7

Electrical safety

8

Biological safety

8

Chemical safety

9

Mechanical

9

Specs for DS96

10

Chapter 1: DropSense96 at a glance

11

DropSense96 Overview

11

Consumables

12

External Characteristics

17

Chapter 2: Software

19

DropQuant

19

Computer requirements

19

Installation/Updates

19

Login

19

Home screen

20

New Measurement

21

Plate Layout

22

Import

24

Measurement

26

Chapter 3: Applications

27

Nucleic Acids

27

Oligo measurements

28

Proteins

29

General UV/Vis and Standard Curve

31

Standard curves

31

Chapter 4: Interpreting data

38

DropQuant DataViewer

38

DataViewer results window

39

96-Well Display

40

Column Display

40

Single Well Display

41

Report Display

42

Chapter 5: Exporting data

44

Export Results

44

Export Outliers

45

Export Normalization

45

2

Quick Export

47

Chapter 6: Software - extra functionalities

48

Users

48

Bar code scanner

48

Available wells database

51

Chapter 7: Software - advanced settings

52

The system info and settings window

52

System info

52

Edit Settings

53

Data viewer extra functions

56

Chapter 8: Diagnostic and calibration tools

57

Optical calibration

57

Potassium dichromate check

58

Quick Check

59

Generate status zip file

60

Chapter 9: Troubleshooting

61

Solving an issue

61

FAQ list

61

Annex A: Theory

65

DNA/RNA concentration and purity determination 65

Protein concentration and purity determination 66

General UV/Vis spectrophotometry

67

Principle of standard curve measurements

67

Annex B: Maintenance

69

Cleaning the DropSense 96

69

Maintenance

69

Annex X: Glossary

70

This manual is published by TRINEAN NV/SA. Questions or comments regarding the content of this manual can be directed to the address below or to your TRINEAN representative.

TRINEAN NV/SA Dulle Grietlaan 17/3 B-9050 Gentbrugge, Belgium Tel. 0032 9 2727535

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Fax. 0032 9 2727539 info@ ?DropSense 96 and ?DropPlate16/96 are trademarks of TRINEAN NV/SA. This document is the copyright of TRINEAN and must not be copied or reproduced in any form without prior consent. TRINEAN reserves the right to make technical improvements to this equipment and documentation without prior notice as part of a continuous program of product development. This manual supersedes all previous editions.

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Trinean warranty statement

Warranty and Special Provisions Limited Warranty TRINEAN products are fully guaranteed for one year against defects in parts, materials, and workmanship. Defective parts and materials will be replaced or, at the discretion of TRINEAN, repaired at no charge for a period of one year, provided that the products are utilized and maintained in accordance with the instructions in the applicable operating and servicing manual, and provided further that the products have not, as determined solely by TRINEAN, been subject to misuse or abuse by the Customer or other parties unrelated to TRINEAN. TRINEAN makes no warranty, expressed or implied, as to the fitness of any product for any particular purposes other than those purposes described in the applicable operating and servicing manual, nor does TRINEAN make any other warranty, whether expressed or implied, including merchantability, other than those appearing on the face hereof.

Where TRINEAN guarantees any product, whether under this Warranty or as a matter of law, and there is a breach of such guarantee, the Customer's only and exclusive remedy shall be the replacement of defective parts and materials, as described above. This shall be the limit of TRINEANS' liability.

Trinean will provide spare parts of DS-series instruments and related items will be made available at least 7 years, directly or through third parties (e.g. distributors).

Furthermore, TRINEAN shall not be liable for incidental or consequential damages. Failure of the Customer to notify TRINEAN of a claimed defect by registered mail within thirty days of the discovery thereof shall constitute a waiver of any claim for breach of warranty. When a product is required by TRINEAN to be installed by a TRINEAN certified engineer, the period of this Warranty shall begin on the date of such installation, provided however, that any use of the product prior to such installation shall, at sole election of TRINEAN, void this Warranty. When installation by TRINEAN personnel is not required, the period of this Warranty shall begin on the date of shipment from TRINEAN. The period of this Warranty shall begin as described above whether or not the product has been installed pursuant to a purchase order, and any trial period shall be deducted from the Warranty period that would otherwise apply under a subsequent placed purchase order for that product.

Limitation of Liability. Notwithstanding anything to the contrary contained herein, the Seller (whether by reason of breach of warranty, breach of contract, tort, or otherwise), including without limitation under any indemnification provision contained herein, shall be limited to replacement of goods returned to TRINEAN which are shown to TRINEANS' reasonable satisfaction to have been nonconforming or to refund the purchase price, or, if not paid, to a credit amount of the purchase price therefore.

THE FOREGOING WARRANTIES ARE EXCLUSIVE AND A RE GIVEN AND ACCEPTED IN LIEU OF ANY AND ALL OTHER WARRANTIES, EXPRESS OR IMPLIED, INCLUDING WITHOUT LIMITATION, THE IMPLIED WARRANTY OF MECHANTABILITY AND THE IMPLIED WARRANTY OF FITNESS FOR A PARTICULAR PURPOSE. NEITHER PARTY SHALL BE LIABLE TO THE OTHER FOR ANY INCIDENTAL, INDIRECT, SPECIAL, OR CONSEQUENTIAL DAMAGES.

Warranty statements may differ in specific countries.

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Introduction

Thank you for purchasing the DropSense96. This is a unique system for the quantification of microliter samples, consisting of DropPlate-Disposables, a DropSense96 instrument and DropQuant software. This manual is intended for all DropSense users, and it describes the installation, operation and measurement procedures. An electronic copy of this manual can be opened from the DropQuant program by clicking the Help button.

Warning: Situations that can damage the instrument or your experiment.

Caution: Situations that can potentially affect the results of your measurements.

Note: Notes, usage tips, or additional information.

Warning

The DropSense system is designed for UV/Vis quantification of small samples. Using the system for other applications doesn't guarantee the user's safety and proper functioning of the instrument. This instrument is intended to be used by persons having a basic knowledge of UV/Vis spectroscopy and the handling of nucleic acids and protein samples. Before starting the installation or use of the system, read this manual carefully and make sure to understand the instructions to avoid safety issues. Keep the manual close to the instrument for reference. The content of this manual is subject to change without prior notice.

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Safety Information

Before using the DropSense 96 system, it is essential that you read this user manual carefully and pay particular attention to the safety information. The instructions and safety information in the user manual must be followed to ensure safe operation of the DropSense 96 and to maintain the DropSense 96 in a safe condition. The following types of safety information appear throughout this user manual. Details about these circumstances are given in a box the one below. The advice given in this manual is intended to supplement, not supersede, the normal safety requirements prevailing in the user's country.

Handling and positioning Always make sure the instrument is placed on a steady surface, allowing easy access to its back. It is extremely important that both the power button and the power cable at the back are always easily accessible. Before moving/repositioning the instrument, always make sure that there's enough space for that. Failing to provide adequate work space can cause material damage or injury to the handler.

Risk of personal injury When moving the instrument, always make sure to use a 2 person lift as the apparatus weights more than 18kg.

Risk of personal injury and material damage [W1] Improper use of the DropSense 96 may cause personal injuries or damage to the instrument. The DropSense 96 must only be operated by appropriately trained and experienced personnel. Servicing of the DropSense 96 must only be performed by Trinean trained service personnel or service technicians of an authorized agent. Using the apparatus in a manner which is not specified in this user manual can impair the protection provided by the equipment.

Risk of personal injury and material damage [W2] Do not attempt to move the DropSense 96 during operation.

Explosive atmosphere [W3] The DropSense 96 is not designed for use in an explosive atmosphere.

Damage to the instrument [C1] Direct sunlight may bleach parts of the instrument and cause damage to plastic parts. The DropSense 96 must be located out of direct sunlight.

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Damage to the instrument [C2] Avoid spilling water or chemicals onto the DropSense 96. Damage caused by water or chemical spillage will void your warranty. In case of emergency, switch off the DropSense 96 at the power switch and unplug the power supply from the power outlet.

Moveable parts While operating the instrument, please beware of any moveable parts.

Electrical safety

Note: Disconnect the line power outlet before servicing.

Electrical hazard [W4] Any interruption of the protective conductor (earth/ground lead) inside or outside the instrument or disconnection of the protective conductor terminal is likely to make the instrument dangerous. Intentional interruption is prohibited. Lethal voltages inside the instrument When the instrument is connected to line power, terminals may be live. Opening covers or removing parts is likely to expose live parts.

Electrical hazard [W4] Only use the provided power supply and supply cord. For replacements contact your local distributor. Use of wrong power supply may cause fire due to overheating.

To ensure satisfactory and safe operation of the DropSense 96 follow the guidelines below: The line power cord must be connected to a line power outlet that has a protective conductor (earth/ground). If the instrument becomes electrically unsafe, prevent other personnel from operating it, and contact your local distributor or Trinean Service support. The instrument may be electrically unsafe when: ? The line power cord appears to be damaged. ? It has been stored for a prolonged period of time in conditions which are outside of the normal storage conditions. ? It has been subjected to severe transport stresses.

Biological safety

Samples Samples may contain infectious agents. You should be aware of the health hazard presented by such agents, and should use, store, and dispose of such samples according to the required safety regulations.

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