SSR PCR Condition--- ABI 3100-BRC Machine (capillary system)



SSR PCR Condition--- ABI 3730-BRC Machine (capillary system)

Ying/Rebecca

(6-18-03)

I> PCR Condition for ABI 3730-BRC Machine (capillary system)

| |Stock Concentration |One Reaction-one primer |

|ABI GeneScan Tm 500 LIZTm Size standard |0.05 ul |5 ul |

|(4C) | | |

|dH2O |0.45 ul |45ul |

|ABI Hi-Di Formamide (4C) |9 ul |900 ul |

|PCR product |0.5 ul | |

Use the mix for 9.5 ul/ well, add 0.5 ul PCR product/well for 96 well plate format.

b) Denature: After step a), denature sample in 95 for 10 mins, then cool the sample in ice pad.- Using MJ Research PTC-100, program “Denature”.

IV. Prepare Sample Sheet for BRC, there is a blank excel sheet for BRC, fill the blank sheet then save as a text only file in sharedrive.

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