Supporting information
Supporting information
Supporting Figure (S1~S5, S7~S10) and Table (S6)
[pic]
S1 The positions of the identified protein spots, including Anx7 (c298).
Representative whole proteome of the RA synoviocytes (left). The ProQ-stained 2-DE gels, which detected phosphoproteins, were further stained with Sypro Ruby to detect whole proteins. Arrowheads show protein spots which were subsequently identified by mass spectrometric analysis. Right panels are magnified images of dot-lined rectangle area in the left panel of Fig. 1B (top; phosphoproteins, bottom; whole proteins).
S2 Phosphorylation intensity of each identified protein in RA- and OA- synoviocyte extracts.
In the identified phosphoprotein spots (ribosomal protein P0, ATP synthese, NDK, lamin B1, and similar to SCP2) shown in Table1 (except Anx7), the intensity of phosphoprotein was compared between the RA group (n=5) and the OA group (n=5). The bars indicate the means of the intensity in each group. In each panel, the means level in the OA samples is defined as 100%. *p ................
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