Billings Clinic
`June
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|Volume 9 , Number 2 | |
|April 2015 |New Testing |
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|The Laboratory Communiqué is a quarterly |Norovirus PCR |
|publication released by Billings Clinic | |
|Laboratory Services as an informational tool|Norovirus is a highly contagious virus and is the most common cause of viral gastroenteritis worldwide. Noroviruses can be |
|for medical staff and laboratorians. |transmitted from an infected person, contaminated food or water, or by touching contaminated surfaces. Norovirus outbreaks |
| |require immediate implementation of targeted infection control procedures. |
|In This Issue | |
|New Tests |Noroviruses are single stranded RNA, non-enveloped viruses in the genus Norovirus, family Caliciviridae, which cause acute |
| |gastroenteritis in humans. Norovirus can be classified into five different genogroups of which genogroup I (GI) and genogroup|
|Norovirus PCR |II (GII) cause the majority of the infections in humans. |
| | |
|Test Number: 9347 |The Norovirus PCR Assay is a qualitative in-vitro diagnostic test for the identification and differentiation of Norovirus |
|CPT Code: 87798 X2 |genogroup I and genogroup II RNA from raw or unpreserved stool specimens collected from individuals with symptoms of acute |
|Testing: Daily |gastroenteritis. The Xpert Norovirus Assay is intended as an aid in the diagnosis of Norovirus |
| | |
| |Specimen: |
| | |
| |Raw or unpreserved unformed stool specimen in a clean preservative-free container. Store the specimen at 2-8°C. The specimen|
| |is stable for up to two days when stored at 2-8°C. If expected transport time to the laboratory is more than two days, freeze|
| |the specimen at -20°C. |
| | |
| |Interpretation: |
| | |
| |Norovirus genogroup I RNA sequence is detected |
| |Norovirus genogroup II RNA sequence is detected |
| |Norovirus genogroup I and Norovirus genogroup II RNA sequences are detected |
| |Norovirus genogroup I and genogroup II target RNA sequences are not detected |
| |Invalid (Presence or absence or the target RNA cannot be determined) |
| | |
| | |
| |CDIFF GDH |
| | |
| |Clostridium difficile acts as an opportunistic pathogen of the intestinal tract in response to antibiotic induced depletion of|
|CDIFF GDH |the normal flora. Clostridium difficile disease can evidence either from an asymptomatic carrier state or nosocomial infection|
| |following antibiotic treatment. Clostridium difficile is a causative agent of two intestinal disorders, antibiotic associated |
|Test Number: 9681 |diarrhea and antibiotic associated pseudo-membranous colitis. Risk groups include patients treated with a wide range of |
|CPT Code: 87449 |antibiotics including, but not limited to clindamycin, ampicillin, and cephalosporins, cancer patients undergoing |
|Testing: Daily |chemotherapy, and patients on wards with ongoing epidemics. |
| | |
| |The ImmunoCard® C. difficile GDH assay is a rapid EIA test used to detect the presence of Clostridium difficile. The assay |
| |detects the presence of the enzyme, glutamate dehydrogenase (GDH), which is produced by all Clostridium difficile organisms. |
| |GDH is also produced by other bacteria. The sensitivity of GDH antigen detection makes it an excellent screening test and the |
|RapidTEG |assay is now part of our CDIFF testing algorithm. |
| | |
|Test Number: 9669 |The ImmunoCard® C. difficile GDH Assay has a 99.6% negative predictive value for early, accurate detection. A positive result |
|CPT Codes: 85347, |obtained with the ImmunoCard® C. difficile GDH assay will reflex to the Xpert C.difficile PCR for confirmation of C. |
|85384, 85576 |difficile. |
|Testing: 24/7 | |
| |Specimen: |
| | |
| |Raw or unpreserved unformed stool specimen in a clean preservative-free container. Store the specimen at 2-8°C until tested. |
| |The specimen is stable for up to 72 hours when stored at 2-8°C. If expected transport time to the laboratory is more than two|
|Basic TEG |days, freeze the specimen at -20°C. |
| | |
|Test Number: 9678 |Interpretation: |
|CPT Codes: 85347, | |
|85384, 85576 |GDH Negative (No further testing will be performed) |
|Testing: 24/7 |GDH Positive (Reflex to the CDIFF PCR for confirmation) |
| |Only Reflex PCR test result reported. |
| | |
| |TEG (Thrombelastograph) |
| | |
|Platelet Mapping |Effective January 5, 2015, Laboratory Services went live with 3 new Hemostasis tests, Basic TEG, Rapid TEG and Platelet |
| |Mapping. All three tests are performed on the Haemonetics Thromboelastograph (TEG) instruments in the laboratory. These tests |
|Test Number: 9670 |are unique in that the test is performed in the Laboratory but the real-time tracing of the test is viewed by the ordering |
|CPT Codes: 85576 |physician via special software installed in the Emergency Department and ICU. During the Go-Live, Haemonetics Technical |
|Testing: M-F |Support Staff were on-site to provide training to the physicians on the clinical interpretation of the tests. Clinical |
|8AM-2:30PM |interpretations will not be made by the Laboratory staff or Pathology physicians. |
| | |
| |The TEG analyzer records the viscoelastic changes in a whole blood sample as the sample clots, retracts and/or lyses. The |
| |enzymatic system is expressed by SP (split point-initial fibrin formation), R (reaction time to coagulation factors), K |
| |(kinetics) and Angle (fibrinogen functionality). For Rapid TEG™, TEGACT (activated clotting time). The platelet system is |
| |expressed by MA (maximum amplitude-true platelet function) and G (clot strength). The lytic system is expressed by LY30 (lysis|
| |30 minutes after MA) and EPL (estimated % to lysis) |
|Test Updates | |
|Thrombin Time-Normal Range change |The ordering test information is: |
| | |
| |Rapid TEG or Basic TEG |
|APTT interpretative remark for Heparin | |
|Therapeutic Range |TEG offers a nearer to real-time assessment of a patient’s hemostasis during a severe trauma and/or a massive hemorrhage. The |
| |TEG can be used to optimize blood component therapy during the management of massive transfusions and the resuscitation of |
| |severe trauma patients. |
|APTT and Heparin Critical Values | |
| |Specimen: Citrated whole blood (blue top tube) |
| |Testing: 24/7 |
|Hgb A1c Normal Range |Lab notified: Emergency Department will notify the lab via the TEG pager |
| |when a Rapid TEG is required |
| | |
| |Platelet Mapping |
|Celebrations | |
| |Platelet Mapping is utilized to monitor platelet function by assessing the degree of inhibition and/or resistance to patients |
|Lab Week 2015 |receiving platelet inhibition drugs for risk of ischemic events affecting the thromboxane A2-(TxA2) pathway (such as aspirin),|
|April 19-25 |ADP inhibitor (such as Clopidogrel, Ticlid) and other GPIIb/IIIa inhibitors (such as Reopro, Aggrastat, Integrilin) including |
| |Persantine, Toradol and Pletal. It can also aid in the detection of any pathological inhibition due to the receptors mentioned|
| |above. |
| | |
| | Specimen: 2 Samples, Citrated whole blood (blue top) and Heparinized |
| | whole blood (green top tube-no gel) |
| | Testing: M-F, 8AM to 2:30PM |
| | Lab notified: Scheduled with Lab prior to patient blood draw |
| | |
| |For further questions, please contact Kathy Johnson, Laboratory Supervisor at 4644 or Ron Linfesty, MD in Pathology at 4069. |
| | |
| |Test Updates |
| |Thrombin Time |
| |The Reference Range for the Thrombin Time was changed effective February 6, 2015. |
| |Old Range: 16.4 – 22.3 seconds |
|[pic] |New Range: 14.4 – 25.5 seconds |
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|Laboratory Services Contact Us | |
|(406) 657-4060 | |
|(866) 232-2522 |APTT Interpretative Remark |
| | |
|Director/Pathologist: |The Interpretative remark for PTT was updated for the new lot of APTT reagent. |
|Jeffrey Smith, MD |Old Heparin Therapeutic Range: 51-107 seconds |
| |New Heparin Therapeutic Range: 69 -134 seconds |
|Lab Director: | |
|Mark Lubbers, MT ASCP |APTT and Heparin Critical Value Changes |
| | |
|Lab Manager: |APTT critical value is now 149 seconds |
|Sheilah Frazier MT ASCP |UF Heparin critical value is now >0.8 IU/mL |
| | |
|Technical Consultant: |HgbA1c Normal Range |
|Joni Gilstrap, MT ASCP |The Reference Range has been changed due to the installation of a new TOSOH G8 Instrument. |
|Extension 4046 |Old Range: 4.2 – 5.8% |
| |New Range: 4.0 - 6.0% |
|Supervisor |2015 Lab Week |
|Kathy Johnson MT ASCP |It will be time to celebrate Lab Week, April 19-25, 2015, and honor your laboratory staff for being a vital part of the lab |
|Extension 4644 |community! |
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|Supervisor |For more information about Billings Clinic Laboratory please call (406) 657-4060. . |
|Rebecca Schulz | |
|Extension 4861 | |
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|Laboratory Marketing Coordinator | |
|Jena DeVries | |
|Extension 4888 | |
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