Online Appendix for the following JACC article

Adolescence serum samples were taken in 1980 and stored in -20°C. These samples were analyzed in 2005 using the same method as in 2001. During the storage, the samples were not thawed or re-frozen.(7) Coronary Artery Calcium Scoring. CT scans were carried out at 3 study locations: Turku, Tampere and Kuopio. ................
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