Research and Development

Agrobacterium cells carrying a binary vector are grown for 24 h in Luria-Bertaini (LB) media supplement with the appropriate antibiotics on an orbital shaker at 28 ºC and 250 rpm. The cells are pelletted by centrifugation at 4000 rpm (1,992 RCF) for 30 min and then re-suspended in an Agrobacterium MS-IM containing 200 ul AS at an OD600nm of 0 ... ................
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