Answers to All Questions and Problems
Answers to All Questions and Problems
Chapter 1
1.1 In a few sentences, what were Mendel's key ideas about inheritance?
ANS: Mendel postulated transmissible factors--genes--to explain the inheritance of traits. He discovered that genes exist in different forms, which we now call alleles. Each organism carries two copies of each gene. During reproduction, one of the gene copies is randomly incorporated into each gamete. When the male and female gametes unite at fertilization, the gene copy number is restored to two. Different alleles may coexist in an organism. During the production of gametes, they separate from each other without having been altered by coexistence.
1.2 Both DNA and RNA are composed of nucleotides. What molecules combine to form a nucleotide?
ANS: Each nucleotide consists of a sugar, a nitrogen-containing base, and a phosphate.
1.3 Which bases are present in DNA? Which bases are present in RNA? Which sugars are present in each of these nucleic acids?
ANS: The bases present in DNA are adenine, thymine, guanine, and cytosine; the bases present in RNA are adenine, uracil, guanine, and cytosine. The sugar in DNA is deoxyribose; the sugar in RNA is ribose.
1.4 What is a genome?
ANS: A genome is the set of all the DNA molecules that are characteristic of an organism. Each DNA molecule forms one chromosome in a cell of the organism.
1.5 The sequence of a strand of DNA is ATTGCCGTC. If this strand serves as the template for DNA synthesis, what will be the sequence of the newly synthesized strand?
ANS: TAACGGCAG
1.6 A gene contains 141 codons. How many nucleotides are present in the gene's coding sequence? How many amino acids are expected to be present in the polypeptide encoded by this gene?
ANS: There are 3 ? 141 = 423 nucleotides in the gene's coding sequence. Its polypeptide product will contain 141 amino acids.
1.7 The template strand of a gene being transcribed is CTTGCCAGT. What will be the sequence of the RNA made from this template?
ANS: GAACGGUCT
1.8 What is the difference between transcription and translation?
ANS: Transcription is the production of an RNA chain using a DNA chain as a template. Translation is the production of a chain of amino acids--that is, a polypeptide--using an RNA chain as a template.
1.9 RNA is synthesized using DNA as a template. Is DNA ever synthesized using RNA as a template? Explain.
ANS: Sometimes, DNA is synthesized from RNA in a process called reverse transcription. This process plays an important role in the life cycles of some viruses.
1.10 The gene for a-globin is present in all vertebrate species. Over millions of years, the DNA sequence of this gene has changed in the lineage of each species. Consequently, the amino acid sequence of a-globin has also changed in these lineages. Among the 141 amino acid positions in this polypeptide, human a-globin differs from shark a-globin in 79 positions; it differs from carp a-globin in 68 and from cow a-globin in 17. Do these data suggest an evolutionary phylogeny for these vertebrate species?
ANS:
The human and cow a-globins are least different; therefore, on the assumption that differences in a-globin reflect the degree of phylogenetic relationship, the human and the cow are the most closely related organisms among those mentioned. The next closest "relative" of humans is the carp, and the most distant relative is the shark.
1.11 Sickle-cell anemia is caused by a mutation in one of the codons in the gene for b-globin; because of this mutation, the sixth amino acid in the b-globin polypeptide is a valine instead of a glutamic acid. A less severe type of anemia is caused by a mutation that changes this same codon
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to one specifying lysine as the sixth amino acid in the b-globin polypeptide. What word is used to describe the two mutant forms of this gene? Do you think that an individual carrying these two mutant forms of the b-globin gene would suffer from anemia? Explain.
ANS:
The two mutant forms of the b-globin gene are properly described as alleles. Because neither of the mutant alleles can specify a "normal" polypeptide, an individual who carries each of them would probably suffer from anemia.
1.12 Hemophilia is an inherited disorder in which the bloodclotting mechanism is defective. Because of this defect, people with hemophilia may die from cuts or bruises, especially if internal organs such as the liver, lungs, or kidneys have been damaged. One method of treatment involves injecting a blood-clotting factor that has been purified from blood donations. This factor is a protein encoded by a human gene. Suggest a way in which modern genetic technology could be used to produce this factor on an industrial scale. Is there a way in which the inborn error of hemophilia could be corrected by human gene therapy?
ANS: The gene for the human clotting factor could be isolated from the human genome and transferred into bacteria, which could then be grown in vats to produce large amounts of the gene's protein product. This product could be isolated from the bacteria, purified, and then injected into patients to treat hemophilia. Another approach would be to transfer a normal copy of the clotting factor gene into the cells of people who have hemophilia. If expressed properly, the transferred normal gene might be able to compensate for the mutant allele these people naturally carry. For this approach to succeed, the normal clotting factor gene would have to be transferred into the cells that produce clotting factor, or into their precursors.
Chapter 2
2.1 Carbohydrates and proteins are linear polymers. What types of molecules combine to form these polymers?
ANS: Sugars combine to form carbohydrates; amino acids combine to form proteins.
2.2 All cells are surrounded by a membrane; some cells are surrounded by a wall. What are the differences between cell membranes and cell walls?
ANS: Cell membranes are made of lipids and proteins; they have a fluid structure. Cell walls are made of more rigid materials such as cellulose.
2.3 What are the principal differences between prokaryotic and eukaryotic cells?
ANS: In a eukaryotic cell, the many chromosomes are contained within a membrane-bounded structure called the nucleus; the chromosomes of prokaryotic cells are not contained within a special subcellular compartment.
Eukaryotic cells usually possess a well-developed internal system of membranes and they also have membranebounded subcellular organelles such as mitochondria and chloroplasts; prokaryotic cells do not typically have a system of internal membranes (although some do), nor do they possess membrane-bounded organelles.
2.4 Distinguish between the haploid and diploid states. What types of cells are haploid? What types of cells are diploid?
ANS: In the haploid state, each chromosome is represented once; in the diploid state, each chromosome is represented twice. Among multicellular eukaryotes, gametes are haploid and somatic cells are diploid.
2.5 Compare the sizes and structures of prokaryotic and eukaryotic chromosomes.
ANS: Prokaryotic chromosomes are typically (but not always) smaller than eukaryotic chromosomes; in addition, prokaryotic chromosomes are circular, whereas eukaryotic chromosomes are linear. For example, the circular chromosome of E. coli, a prokaryote, is about 1.4 mm in circumference. By contrast, a linear human chromosome may be 10?30 cm long. Prokaryotic chromosomes also have a comparatively simple composition: DNA, some RNA, and some protein. Eukaryotic chromosomes are more complex: DNA, some RNA, and a lot of protein.
2.6 With a focus on the chromosomes, what are the key events during interphase and M phase in the eukaryotic cell cycle?
ANS: During interphase, the chromosomes duplicate. During M phase (mitosis), the duplicated chromosomes, each consisting of two identical sister chromatids, condense (a feature of prophase), migrate to the equatorial plane of the cell (a feature of metaphase), and then split so that their constituent sister chromatids are separated into different daughter cells (a feature of anaphase); this last process is called sister chromatid disjunction.
2.7 Which typically lasts longer, interphase or M phase? Can you explain why one of these phases lasts longer than the other?
ANS: Interphase typically lasts longer than M phase. During interphase, DNA must be synthesized to replicate all the chromosomes. Other materials must also be synthesized to prepare for the upcoming cell division.
2.8 In what way do the microtubule organizing centers of plant and animal cells differ?
ANS: The microtubule organizing centers of animal cells have distinct centrosomes, whereas the microtubule organizing centers of plant cells do not.
2.9 Match the stages of mitosis with the events they encompass: Stages: (1) anaphase, (2) metaphase, (3) prophase, and (4) telophase. Events: (a) reformation of the nucleolus, (b) disappearance of the nuclear membrane,
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(c) condensation of the chromosomes, (d) formation of the mitotic spindle, (e) movement of chromosomes to the equatorial plane, (f) movement of chromosomes to the poles, (g) decondensation of the chromosomes, (h) splitting of the centromere, and (i) attachment of microtubules to the kinetochore.
ANS: (1) Anaphase: (f), (h); (2) metaphase: (e), (i); (3) prophase: (b), (c), (d); (4) telophase: (a), (g).
2.10 Arrange the following events in the correct temporal sequence during eukaryotic cell division, starting with the earliest: (a) condensation of the chromosomes, (b) movement of chromosomes to the poles, (c) duplication of the chromosomes, (d) formation of the nuclear membrane, (e) attachment of microtubules to the kinetochores, and (f) migration of centrosomes to positions on opposite sides of the nucleus.
ANS: (c), (f), (a), (e), (b), (d).
2.11 In human beings, the gene for b-globin is located on chromosome 11, and the gene for a-globin, which is another component of the hemoglobin protein, is located on chromosome 16. Would these two chromosomes be expected to pair with each other during meiosis? Explain your answer.
ANS: Chromosomes 11 and 16 would not be expected to pair with each other during meiosis; these chromosomes are heterologues, not homologues.
2.12 A sperm cell from the fruit fly Drosophila melanogaster contains four chromosomes. How many chromosomes would be present in a spermatogonial cell about to enter meiosis? How many chromatids would be present in a spermatogonial cell at metaphase I of meiosis? How many would be present at metaphase II?
ANS: There are eight chromosomes in a Drosophila spermatogonial cell about to enter meiosis. There are 16 chromatids in a Drosophilia spermatogonial cell at metaphase I of meiosis. There are eight chromatids in a Drosophilia cell at metaphase II of meiosis.
2.13 Does crossing over occur before or after chromosome duplication in cells going through meiosis?
ANS: Crossing over occurs after chromosomes have duplicated in cells going through meiosis.
2.14 What visible characteristics of chromosomes indicate that they have undergone crossing over during meiosis?
ANS: The chiasmata, which are visible late in prophase I of meiosis, indicate that chromosomes have crossed over.
2.15 During meiosis, when does chromosome disjunction occur? When does chromatid disjunction occur?
ANS: Chromosome disjunction occurs during anaphase I. Chromatid disjunction occurs during anaphase II.
2.16 In Arabidopsis, is leaf tissue haploid or diploid? How many nuclei are present in the female gametophyte?
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How many are present in the male gametophyte? Are these nuclei haploid or diploid?
ANS: Leaf tissue is diploid. The female gametophyte contains eight identical haploid nuclei. The male gametophyte contains three identical haploid nuclei.
2.17 From the information given in Table 2.1 in this chapter, is there a relationship between genome size (measured in base pairs of DNA) and gene number? Explain.
ANS: Among eukaryotes, there does not seem to be a clear relationship between genome size and gene number. For example, humans, with 3.2 billion base pairs of genomic DNA, have about 20,500 genes, and Arabidopsis plants, with about 150 million base pairs of genomic DNA, have roughly the same number of genes as humans. However, among prokaryotes, gene number is rather tightly correlated with genome size, probably because there is so little nongenic DNA.
2.18 Are the synergid cells in an Arabidopsis female gametophyte genetically identical to the egg cell nestled between them?
ANS: Yes.
2.19 A cell of the bacterium Escherichia coli, a prokaryote, contains one chromosome with about 4.6 million base pairs of DNA comprising 4288 protein-encoding genes. A cell of the yeast Saccharomyces cerevisiae, a eukaryote, contains about 12 million base pairs of DNA comprising 6268 genes, and this DNA is distributed over 16 distinct chromosomes. Are you surprised that the chromosome of a prokaryote is larger than some of the chromosomes of a eukaryote? Explain your answer.
ANS: It is a bit surprising that yeast chromosomes are, on average, smaller than E. coli chromosomes because, as a rule, eukaryotic chromosomes are larger than prokaryotic chromosomes. Yeast is an exception because its genome-- not quite three times the size of the E. coli genome--is distributed over 16 separate chromosomes.
2.20 Given the way that chromosomes behave during meiosis, is there any advantage for an organism to have an even number of chromosome pairs (such as Drosophila does), as opposed to an odd number of chromosome pairs (such as human beings do)?
ANS: No, there is no advantage associated with an even number of chromosomes. As long as the chromosomes come in pairs, they will be able to synapse during prophase I and then disjoin during anaphase I to distribute the genetic material properly to the two daughter cells.
2.21 In flowering plants, two nuclei from the pollen grain participate in the events of fertilization. With which nuclei from the female gametophyte do these nuclei combine? What tissues are formed from the fertilization events?
ANS: One of the pollen nuclei fuses with the egg nucleus in the female gametophyte to form the zygote, which then
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develops into an embryo and ultimately into a sporophyte. The other genetically functional pollen nucleus fuses with two nuclei in the female gametophyte to form a triploid nucleus, which then develops into a triploid tissue, the endosperm; this tissue nourishes the developing plant embryo.
2.22 The mouse haploid genome contains about 2.9 ? 109 nucleotide pairs of DNA. How many nucleotide pairs of DNA are present in each of the following mouse cells: (a) somatic cell, (b) sperm cell, (c) fertilized egg, (d) primary oocyte, (e) first polar body, and (f) secondary spermatocyte?
ANS: (a) 5.8 ? 109 nucleotide pairs (np); (b) 2.9 ? 109 np; (c) 5.8 ? 109 np; (d) 11.6 ? 109 np; (e) 5.8 ? 109 np; and (f) 5.8 ? 109 np
2.23 Arabidopsis plants have 10 chromosomes (five pairs) in their somatic cells. How many chromosomes are present in each of the following: (a) egg cell nucleus in the female gametophyte, (b) generative cell nucleus in a pollen grain, (c) fertilized endosperm nucleus, and (d) fertilized egg nucleus?
ANS: (a) 5, (b) 5, (c) 15, (d) 10.
Chapter 3
3.1 On the basis of Mendel's observations, predict the results from the following crosses with peas: (a) a tall (dominant and homozygous) variety crossed with a dwarf variety; (b) the progeny of (a) self-fertilized; (c) the progeny from (a) crossed with the original tall parent; (d) the progeny of (a) crossed with the original dwarf parent.
ANS: (a) All tall; (b) 3/4 tall, 1/4 dwarf; (c) all tall; (d) 1/2 tall, 1/2 dwarf.
3.2 Mendel crossed pea plants that produced round seeds with those that produced wrinkled seeds and self-fertilized the progeny. In the F2, he observed 5474 round seeds and 1850 wrinkled seeds. Using the letters W and w for the seed texture alleles, diagram Mendel's crosses, showing the genotypes of the plants in each generation. Are the results consistent with the Principle of Segregation?
ANS: Round (WW) ? wrinkled (ww) F1 round (Ww); F1 self-fertilized F2 3/4 round (2 WW; 1 Ww), 1/4 wrinkled (ww). The expected results in the F2 are 5493 round, 1831 wrinkled. To compare the observed and expected results, compute c2 with one degree of freedom; (5474 - 5493)2/5493 = (1850 - 1831)2/1831 = 0.263, which is not significant at the 5% level. Thus, the results are consistent with the Principle of Segregation.
3.3 A geneticist crossed wild, gray-colored mice with white (albino) mice. All the progeny were gray. These progeny were intercrossed to produce an F2, which consisted of 198 gray and 72 white mice. Propose a hypothesis to
explain these results, diagram the crosses, and compare the results with the predictions of the hypothesis.
ANS: The data suggest that coat color is controlled by a single gene with two alleles, C (gray) and c (albino), and that C is dominant over c. On this hypothesis, the crosses are gray (CC) ? albino (cc) F1 gray (Cc); F1 ? F1 3/4 gray (2 CC: 1 Cc), 1/4 albino (cc). The expected results in the F2 are 203 gray and 67 albino. To compare the observed and expected results, compute c2 with one degree of freedom: (198 - 203)2/203 + (67 - 72)2/72 = 0.470, which is not significant at the 5% level. Thus, the results are consistent with the hypothesis.
3.4 A woman has a rare abnormality of the eyelids called ptosis, which prevents her from opening her eyes completely. This condition is caused by a dominant allele, P. The woman's father had ptosis, but her mother had normal eyelids. Her father's mother had normal eyelids.
(a) What are the genotypes of the woman, her father, and her mother?
(b) What proportion of the woman's children will have ptosis if she marries a man with normal eyelids?
ANS: (a) Woman's genotype Pp, father's genotype Pp, mother's genotype pp; (b) ?
3.5 In pigeons, a dominant allele C causes a checkered pattern in the feathers; its recessive allele c produces a plain pattern. Feather coloration is controlled by an independently assorting gene; the dominant allele B produces red feathers, and the recessive allele b produces brown feathers. Birds from a true-breeding checkered, red variety are crossed to birds from a true-breeding plain, brown variety.
(a) Predict the phenotype of their progeny.
(b) If these progeny are intercrossed, what phenotypes will appear in the F2 and in what proportions?
ANS: (a) Checkered, red (CC BB) ? plain, brown (cc bb) F1 all checkered, red (Cc Bb); (b) F2 progeny: 9/16 checkered, red (C- B-), 3/16 plain, red (cc B-), 3/16 checkered, brown (C- bb), 1/16 plain, brown (cc bb).
3.6 In mice, the allele C for colored fur is dominant over the allele c for white fur, and the allele V for normal behavior is dominant over the allele v for waltzing behavior, a form of dis-coordination. Given the genotypes of the parents in each of the following crosses:
(a) Colored, normal mice mated with white, normal mice produced 29 colored, normal, and 10 colored, waltzing progeny
(b) Colored, normal mice mated with colored, normal mice produced 38 colored, normal, 15 colored, waltzing, 11 white, normal, and 4 white, waltzing progeny
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(c) Colored, normal mice mated with white, waltzing mice produced 8 colored, normal, 7 colored, waltzing, 9 white, normal, and 6 white, waltzing progeny.
ANS: (a) colored, normal (CC Vv) ? white, normal (cc Vv) (b) colored, normal (Cc Vv) ? colored, normal (Cc Vv); (c) colored, normal (Cc Vv) ? white, waltzing (cc vv).
3.7 In rabbits, the dominant allele B causes black fur and the recessive allele b causes brown fur; for an independently assorting gene, the dominant allele R causes long fur and the recessive allele r (for rex) causes short fur. A homozygous rabbit with long, black fur is crossed with a rabbit with short, brown fur, and the offspring are intercrossed. In the F2, what proportion of the rabbits with long, black fur will be homozygous for both genes?
ANS: Among the F2 progeny with long, black fur, the genotypic ratio is 1 BB RR: 2 BB Rr: 2 Bb RR: 4 Bb Rr; thus, 1/9 of the rabbits with long, black fur are homozygous for both genes.
3.8 In shorthorn cattle, the genotype RR causes a red coat, the genotype rr causes a white coat, and the genotype Rr causes a roan coat. A breeder has red, white, and roan cows and bulls. What phenotypes might be expected from the following matings and in what proportions?
(a) Red ? red
(b) Red ? roan
(c) Red ? white
(d) Roan ? roan.
ANS: (a) All red; (b) 1/2 red, 1/2 roan; (c) all roan; (d) 1/4 red, 1/2 roan, 1/4 white
3.9 How many different kinds of F1 gametes, F2 genotypes, and F2 phenotypes would be expected from the following crosses:
(a) AA ? aa;
(b) AA BB ? aa bb;
(c) AA BB CC ? aa bb cc?
(d) What general formulas are suggested by these answers?
ANS:
F1 Gametes
F2 Genotypes
(a) 2
3
(b) 2 ? 2 = 4
3 ? 3 = 9
(c) 2 ? 2 ? 2 = 8
3 ? 3 ? 3 = 27
(d) 2n
3n
F2 Phenotypes
2 2 ? 2 = 4 2 ? 2 ? 2 = 8 2n, where n is the number of genes
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3.10 A researcher studied six independently assorting genes in a plant. Each gene has a dominant and a recessive allele: R black stem, r red stem; D tall plant, d dwarf plant; C full pods, c constricted pods; O round fruit, o oval fruit; H hairless leaves, h hairy leaves; W purple flower, w white flower. From the cross (P1) Rr Dd cc Oo Hh Ww ? (P2) Rr dd Cc oo Hh ww,
(a) How many kinds of gametes can be formed by P1?
(b) How many genotypes are possible among the progeny of this cross?
(c) How many phenotypes are possible among the progeny?
(d) What is the probability of obtaining the Rr Dd cc Oo hh ww genotype in the progeny?
(e) What is the probability of obtaining a black, dwarf, constricted, oval, hairy, purple phenotype in the progeny?
ANS: (a) 2 ? 2 ? 1 ? 2 ? 2 ? 2 = 32; (b) 3 ? 2 ? 2 ? 2 ? 3 ? 2 = 144; (c) 2 ? 2 ? 2 ? 2 ? 2 ? 2 = 64; (d) (1/2) ? (1/2) ? (1/2) ? (1/2) ? (1/4) ? (1/2) = 1/128; (e) (3/4) ? (1/2) ? (1/2) ? (1/2) ? (1/4) ? (1/2) = 3/256.
3.11 For each of the following situations, determine the degrees of freedom associated with the c2 statistic and decide whether or not the observed c2 value warrants acceptance or rejection of the hypothesized genetic ratio.
Hypothesized Ratio
Observed-2
(a) 3:1
7.0
(b) 1:2:1
7.0
(c) 1:1:1:1
7.0
(d) 9:3:3:1
5.0
ANS: (a) 1, reject; (b) 2, reject; (c) 3, accept; (d) 3, accept.
3.12 Mendel testcrossed pea plants grown from yellow, round F1 seeds to plants grown from green, wrinkled seeds and obtained the following results: 31 yellow, round; 26 green, round; 27 yellow, wrinkled; and 26 green, wrinkled. Are these results consistent with the hypothesis that seed color and seed texture are controlled by independently assorting genes, each segregating two alleles?
ANS: On the hypothesis, the expected number in each class is 27.5; c2 with three degrees of freedom is calculated as (31 - 27.5)2/27.5 + (26 - 27.5)2/27.5 + (27 - 27.5)2/27.5 + (26 - 27.5)2/27.5 = 0.618, which is not significant at the 5% level. Thus, the results are consistent with the hypothesis of two independently assorting genes, each segregating two alleles.
3.13 Perform a chi-square test to determine if an observed ratio of 30 tall to 20 dwarf pea plants is consistent with an expected ratio of 1:1 from the cross Dd ? dd.
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