Analyses of in vitro non-enzymatic glucosylation of normal ...
) with 10 ul angiotensin I or II (2 mM ) at room temperature for 10 min. ZipTips from Millipore (Billerica, MA) packed with the C18 resin were used to remove the access of Au ion first and then C18 resin bounded peptide and the Au ion-peptide complex were eluted into 5 μl of 50% acetonitrile according to manufacture's instruction. ................
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