Hofmann T, Schmidt J, Ciesielski E, et al. Intein mediated ...

A2 to A9: Antibody fragments anti-CD40 Int N and huFc Int C were mixed in a ratio 3:1 and PTS was activated by 0.5 mM TCEP. Reconstituted antibodies were separated from non-reconstituted antibodies via Ni 2+ beads and further treated with DHAA for reoxidation. Reconstituted anti-CD40 antibodies are indicated at 145.7 kDa under non-reduced ... ................
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