Prepared By - Beckman Coulter
This procedure is valid for the following chemistry analyzers:
|AU400/AU400e |AU640/AU640e |
|AU480 |AU680 |
|AU600 |AU2700/AU5400 |
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PRINCIPLE:
Streptolysin O is a hemolysin produced by group A streptococci. In an infected individual streptolysin O acts as a protein antigen to which the patient mounts an antibody response. A rise in titer begins about 1 week after infection and peaks 2-4 weeks later. In the absence of complications or re-infection, the ASO titer will usually fall to pre-infection levels within 6-12 months. Both clinical and laboratory findings should be correlated in reaching a diagnosis.
INTENDED USE:
System reagent for the quantitative determination of Anti-Streptolysin O antibodies in human serum on the Beckman Coulter AU Clinical Chemistry analyzers.
METHODOLOGY:
Immune complexes formed in solution scatter light in proportion to their size, shape and concentration. Turbidimeters measure the reduction of incidence light due to reflection, absorption or scatter.
In the Beckman Coulter AU system procedure, the rate of decrease in light intensity transmitted (increase in absorbance) through particles suspended in solution is the result of complexes formed during the antigen-antibody reaction.
SPECIMEN:
Patient Preparation:
None required.
|Additional instructions for patient preparation as designated by this laboratory: |
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Type:
Serum, free from hemolysis, is the recommended specimen.
|Additional type conditions as designated by this laboratory: |
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Handling Conditions:
Anti-streptolysin O specimens are stable for several weeks stored at 2 - 8(C. For longer storage, freeze serum at ................
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