Applied Biosystems Veriti Thermal Cycler

Applied Biosystems VeritiTM Thermal Cycler

User Guide

Applied Biosystems VeritiTM Thermal Cycler

User Guide

? Copyright 2008, 2010 Applied Biosystems. All rights reserved. General Laboratory Use. Information in this document is subject to change without notice. Applied Biosystems assumes no responsibility for any errors that may appear in this document. APPLIED BIOSYSTEMS DISCLAIMS ALL WARRANTIES WITH RESPECT TO THIS DOCUMENT, EXPRESSED OR IMPLIED, INCLUDING BUT NOT LIMITED TO THOSE OF MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE. IN NO EVENT SHALL APPLIED BIOSYSTEMS BE LIABLE, WHETHER IN CONTRACT, TORT, WARRANTY, OR UNDER ANY STATUTE OR ON ANY OTHER BASIS FOR SPECIAL, INCIDENTAL, INDIRECT, PUNITIVE, MULTIPLE OR CONSEQUENTIAL DAMAGES IN CONNECTION WITH OR ARISING FROM THIS DOCUMENT, INCLUDING BUT NOT LIMITED TO THE USE THEREOF. NOTICE TO PURCHASER: The VeritiTM Thermal Cycler is covered by US patent claims and corresponding claims in their non-US counterparts. No right is conveyed expressly, by implication, or by estoppel under any other patent claim, such as claims to apparatus, reagents, kits, or methods such as 5' nuclease methods. Further information on purchasing licenses may be obtained by contacting the Director of Licensing, Applied Biosystems, 850 Lincoln Centre Drive, Foster City, California 94404, USA. TRADEMARKS: Applera, Applied Biosystems, AB (Design), ABI PRISM, BigDye, and GeneAmp, are registered trademarks and MicroAmp, VeriFlex, and Veriti are trademarks of Applied Biosystems or its subsidiaries in the U.S. and/or certain other countries. AmpliCycle, AmpliTaq, and AmpliTaq Gold is a registered trademark of Roche Molecular Systems, Inc. All other trademarks are the sole property of their respective owners.

Part Number 4375799 Rev. E 06/2010

Contents

Front Matter

Preface

How to Use This Guide . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . vii How to Obtain More Information . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . viii How to Obtain Support . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . viii

Safety and EMC Compliance Information

Safety Conventions Used in This Document . . . . . . . . . . . . . . . . . . . . . . . . . . . . . xii Symbols on Instruments . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . xiii Safety Labels on Instruments . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . xiv General Instrument Safety . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . xv Chemical Safety . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . xvi Chemical Waste Safety . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . xvii Electrical Safety . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . xvii Physical Hazard Safety . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . xvii Safety and Electromagnetic Compatibility (EMC) Standards . . . . . . . . . . . . . . . xvii

Chapter 1

System Overview

Introducing the VeritiTM Thermal Cycler . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1-2 Using the Touchscreen . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1-3 Menu Overview . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1-6 About Run Methods . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1-8 About Folders . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1-15

Chapter 2

Getting Started

Installing the VeritiTM Thermal Cycler . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2-2 Before You Start . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2-2 Environmental Requirements . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2-3 Materials . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2-3 Unpacking the VeritiTM System . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2-3 Setting Up the VeritiTM System . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2-4 Connecting to a Network Printer . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2-5 Connecting Together Multiple Instruments . . . . . . . . . . . . . . . . . . . . . . . . . 2-5

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