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Bio-energetic modeling of growth and waste production of Nile tilapia (Oreochromis niloticus L.) in recirculation systems.

Dr. M.C.J. Verdegem1, Dr. Ir. A.A. van Dam1, M.Sc. A.A. Cabarcas-Nuñez2 and Dr. L. Oprea3.

1 Fish Culture and Fisheries Group, Department of Animal Sciences, Wageningen University. P.O.Box 338, 6700 AH Wageningen, The Netherlands.

2 Department of Marine Sciences, University of Puerto Rico, P.O.Box 9013. Mayagüez, PR 00681-5000, USA.

3 Fishing and Aquaculture Department, University of Galati “Dunarea de Jos”, Domneasca Str. 47, 6200 Galati, Romania.

A bio-energetic fish growth simulation (FGS) model was developed for Clarias gariepinus and subsequently adjusted for the culture of Oreochromis niloticus, Oncorhynchus mykiss and Colossoma macropomum. The FGS model was extended with a fish waste module (FWM) to calculate the total waste production due to feeding by tilapias grown in indoor recirculation systems. Wastes calculated included the amount of uneaten feed, feces and NH4+ production. The amounts of wastes produced were expressed as g nitrogen (N). The model was calibrated and validated using 3 independent data sets, together comprising 175 aquarium experiments, monitoring growth in all cases and changes in proximate body composition of O. niloticus between stocking and harvesting in 51 cases. Fishes were grown in the individual weight range of 1 – 290 g using 32-54 % protein diets and feeding levels between 5 and 35 g kg-0.8 d-1. The principal read-out parameters for calibration and validation of the model were final weight and final body fat level. Because waste production is the result of the same metabolic processes that lead to protein and fat deposition, it was assumed that waste production was simulated well when protein and fat deposition are. The calibrated model was used to review the effect of feeding level and dietary protein level on N-waste production per kg tilapia produced. Finally, tilapias were grown in 2 different types of recirculation systems and stagnant water ponds, quantifying N-inputs and the amount of N-wastes recovered from each system. The latter was defined as the sum of N-waste discharged (sludge and sludge water drained) and within system accumulation of N-wastes (organic and inorganic nutrients) during culture. After model calibration, the agreement between simulated and observed final weight and body fat level for all data sets was visualized. In recirculation systems different types of N-wastes were estimated well by the model. Care must be taken when applying the model to pond systems. More insight is needed on feeding ecology of tilapias in these systems.

Introduction

It is not possible to grow animals without producing wastes. Only a small part of the nutrient input is retained in the animals raised (Wit de 1992), and farm management will always be partially geared towards improving the nutrient retention efficiency. On average, the animals raised waste 50-85% of the nutrients received. In aquatic systems, these non-retained nutrients or wastes end up in the same water column where the animals live. The moment waste is produced within the water column its constitution and concentration change. For example, some metabolic end products like NH4+ serve directly as nutrients for plants (Syrett 1981, McCarthy 1981), phosphates form insoluble compounds precipitating at the bottom (Boyd 1995) and wastes like feces or uneaten feeds are mineralized (Mohanti et al. 1994). In addition, in outdoor systems extra nutrients are added to the water column through photosynthesis-based processes or N2-fixation (Olah et al. 1994). Consequently, in aquatic systems it is not possible to measure the exact waste production by farmed animals. Only the amounts of waste accumulating on-farm and the amounts of effluents discharged during a production cycle can be measured.

During farm operation, small volatile molecules like CO2, NH3, O2 or N2 are lost from the systems (Addiscott 1995). In areas with a nutrient surplus, the biomass and nutrients lost in this way are no longer a waste management problem for the farmer, even though for example CO2 or NH3 will create environmental problems in farm-dense areas (Kelly et al. 1994). Contrarily, in nutrient deficient areas, farmers should try to minimize nutrient losses. In both cases, it is important to estimate the amount of nutrients lost during production.

In this study, N-waste was defined as the N present in uneaten feed, in feces and in ammonia gill excretion. The objectives were (1) to model accurately waste production by tilapias grown in recirculation systems based on feed input (2) to analyze the effect of feed composition and feeding level on growth and N-waste production, and (3) to compare simulated waste production to the observed waste accumulation and discharge on-farm. The farming systems used were an indoor recirculation systems, an outdoor recirculation systems and stagnant water ponds.

Material and Methods

Fish waste generator model

A bio-energetic fish growth simulation model was developed for Clarias gariepinus (Machiels and Henken, 1986) and subsequently adjusted for the culture of Oreochromis niloticus and Oncorhynchus mykiss (van Dam and Penning de Vries 1995) and Colossoma macropomum (van der Meer and van Dam 1998). For a detailed description of parameters and equations used in the model see van Dam and Penning de Vries (1995). The model was extended with a fish waste module (FWM) to calculate the waste production as a result of feeding for O. niloticus grown in an indoor recirculation system. The types of wastes calculated included uneaten feed, feces and NH4+ production, and were expressed as weight (grams) of N produced in the system during the entire culture period.

The principal read-out parameters for calibration and validation of the model were protein and fat deposition. Because waste production is the result of the same metabolic processes that lead to protein and fat deposition, it was assumed that waste production is simulated well when protein and fat deposition are.

The original model for O. niloticus, written in Professional Dynamo Plus was translated to Turbo Pascal. To see whether the two versions of the model work identically, the data set used by van Dam and Penning de Vries (1995) was used; results were equal.

Digestibility

Commercially formulated diets are composed of quality ingredients with good digestibility. The fractions of protein, fat or carbohydrate digested were determined for a group of tilapias raised in recirculation systems and fed at maintenance level (5 g kg-0.8 d-1) (Table 1), and were different from the original values used by van Dam and Penning de Vries (1995). The digestibility values obtained for recirculation systems were used in the model.

Table 1: Digestibility of nutrients in tilapia diets. Within parenthesis, the proximate composition (on a dry matter basis) of the commercial diet used in the recirculation system is given.

|Proximate composition feed |Digestibility used by van Dam and |Digestibility in recirculation system |

| |Penning de Vries (1995) | |

|Protein (50 %) |0.8 |0.9 |

|Fat (12 %) |0.5 |0.9 |

|Carbohydrate (29 %) |0.5 |0.4 |

The data sets

Three independent data sets were used for calibration and validation (Table 2, data set 1 through 3). Commercial diets were used, ranging in protein level between 28 and 54% protein, with feeding levels in the range of 5 – 32 g kg-0.8 d-1. Data set 4 refers to growth and waste monitoring experiments in ponds (2 ponds), indoor recirculation systems (2) and outdoor recirculation systems (4). Ponds and outdoor recirculation systems were situated on-campus in Puerto Rico, while the Fish Culture and Fisheries Group in the Netherlands operated the indoor recirculation systems.

Table 2: Data sets used for validation and calibration, giving minimum and maximum values for each set of feeding/growth observations.

|Information available |Data set 1 |Data set 2 |Data set 3 |Data set 4 |

| |Calibration |Validation |Validation |Waste analysis |

|Number of feeding/growth trials |34 |17 |124 |8 |

|Fish | | | | |

|Initial number of fish |25-40 |10-23 |7-626 |396-800 |

|Initial individual weight of fish (g) |12-123 |52-180 |1-200 |34-118 |

|Initial fat % |11-12 |4-11 | |4-11 |

|Final number of fish |25-40 |10-23 |7-586 |384-737 |

|Final individual weight of fish (g) |20-204 |60-240 |5-290 |99-186 |

|Final fat % |8-14 |5-10 | |6-10 |

|Feed | | | | |

|% dry matter |91 |91-92 |91 |89-91 |

|% protein |40-50 |38-45 |32-54 |28-47 |

|% fat |14 |9-13 |7-18 |4-6 |

|% carbohydrate |25-35 |23-34 |7-43 |29-50 |

|Daily amount fed (g) |6-209 |0-89 |0-415 |600-2392 |

|Number days of feeding |25-40 |29-68 |5-49 |30-47 |

|Average water temperature |27-28 |27-28 |28 |28 |

Estimating the amount of uneaten feed

The fish growth model developed by van Dam and Pauly (1995) for O. niloticus limited feed intake by O2 availability, the latter being a function of oxygen pressure in the water column and gill surface area. The disadvantage of this method is that the oxygen concentration and temperature in the water column need to be known. Farmers do not record this type of information. Therefore, maximum feed intake was estimated as a function of body weight. Groups of tilapias growing in the weight ranges of 10-50, 40-140 and 60-210 g, respectively were fed different rations in the range of 5 to 35 g kg-0.8 d-1 of diets with a protein level in the range of 40-50% protein on a dry weight basis. These feeding experiments were part of data set 1 (Table 1). To estimate the maximum feed intake for different size groups of tilapia a multiphasic allometric relation was used (Koops and Grossman 1993) (Figure 1):

Y = a1+ b1X –(b1-b2) r ln[1+ e(X-C)/r] (Equation 1)

Where: X = protein ration (g kg-0.8 d-1)

Y = protein growth (g kg-0.8 d-1)

a1, a2 = intercept equation 1 and 2, respectively

b1, b2 = slope equation 1 and 2, respectively

r = smoothness transition between equation 1 and 2 (nearly 0 = abrupt transition, 2 = smooth transition)

C = central point of transition

Figure 1: Multiphasic equation, when b2 = 0, the second part of the multiphasic equation is a horizontal line.

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The parameters a1 and b1 were assumed to be equal for each size group. Parameters were estimated using the non-linear regression procedure of SAS 6.12. The value of C obtained for each size group was plotted against the average geometric mean body weight. The resulting linear relation between body weight and maximum protein intake was incorporated into the model.

Calibration

The parameters used for calibration were the digestibility of protein, fat and carbohydrate and the slope and intercept of the linear regression between body weight and maximum protein intake. Read-out parameters for calibration were final body weight and final body fat content. Agreement between simulated (Y) and observed (X) values was visualized by plotting simulated values against observed values. The relative error (RE) for each simulation was calculated as (Y – X)/((X + Y)/2) (in %) (van der Meer and van Dam 1998). The average relative error (ARE) is the mean of the RE’s.

Validation

Data set 2 and 3 were used for calibration. Only final body weight was used as read-out parameter for data set 3 because no data on body fat composition were available.

Estimation of N-waste production

Using the calibrated model, the amounts of N-waste generated per kg of tilapia produced were calculated considering two situations. In the first simulation 50-g tilapias were fed a daily ration of 15-g kg-0.8 d-1 using feeds with protein levels varying between 15 and 50% of dry matter. In the second simulation, 50-g tilapias were fed a 35% protein diet (% dry matter) at rations varying between 5 to 40 g kg-0.8 d-1.

Comparison of waste production to the amount of waste recovered

The simulated amounts of waste produced by the fish were compared to the amount of waste accumulated in and discharged from the systems during a production cycle of tilapia (data set 4, Table 2). The following systems were included in the analysis:

- indoor recirculation system, 2 growth trials with a 47% protein diet at a feeding level of 12.5 g kg-0.8 d-1.

- stagnant water ponds, 2 growth trials with 28% protein diet at a feeding level of 18.7 kg-0.8 d-1.

- outdoor recirculation systems with a 30% protein diet, and a feeding level of 15.7 kg-0.8 d-1.

- 2 growth trials, with 24 hour solid waste removal.

- 2 growth trials, with 12 hour solid waste removal.

Results

Estimating the amount of uneaten feed

Figure 2 reviews growth of 3 size classes of tilapia in relation to protein ration. The transition point above which growth does not increase further is given by the C values (Table 3).

Figure 2: Growth in relation to protein ration for 3 size groups of tilapia.

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Table 3: Average individual weight of size groups with the corresponding maximum protein intake level and maximum growth rate.

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The maximum protein ration (Y) was calculated as:

Y = -0.032X + 8.041, (Equation 2)

where X = geometric mean body weight (g).

Calibration

Adjusted parameter values as a result of calibration are given in Figure 3 and Table 4. Figure 4, gives plots of observed (X-axis) against simulated values (Y-axis) for data set 1. The uncorrected plots compare observed to simulated values before inclusion of equation 2 into the model. Before correction, without a limitation to feed intake, simulated weights were higher than observed weights, especially for the larger fishes. Simulated fat deposition was higher than observed fat deposition for the higher feeding levels within each size group. The ARE’s for growth and fat deposition were 19.7 and 17.3, respectively. After calibration the ARE’s became 0.0 and –3.2, respectively.

Figure 3: Regression of body weight against maximum dietary protein ration.

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Table 4: Model parameters before and after calibration.

|Parameter |Before calibration |After calibration |

|Protein digestibility |0.9 |0.9 |

|Fat digestibility |0.9 |0.9 |

|Carbohydrate digestibility |0.4 |0.6 |

|Slope regression maximum protein intake against body |-0.0324 |-0.0273 |

|weight | | |

Figure 4: Agreement between observed and simulated values for fish weight and fat content, using uncorrected (before calibration) and corrected parameters (after calibration, Table 4), based on data set 1.

Validation

Validation results are given in Figure 5. For data set 2, for two growth experiments simulated weights were much higher than observed weights, while the opposite was true for fat deposition. For the other experiments in data set 2, simulated values for growth and fat deposition were slightly lower than observed values (ARE –5.9 and –3.9 for weight and fat deposition, respectively). For data set 3, growth was simulated well for fish up to 200 g. For fish reaching a final weight of 200-295 g, observed weight were lower than simulated weights, causing the ARE to be–9.8).

Figure 5: Agreement between observed and simulated values for fish weight and fat content based on data set 2 and 3.

Estimation of N-waste production

The simulated waste production for fish fed feeds with increasing protein levels at 15-g kg-0.8 d-1 is given in Figure 6. The fish reached a maximum weight of 148 g when fed a 45% protein diet. At still higher dietary protein levels, growth decreased. Ammonia excretion was the largest fraction of N-waste, and increased with dietary protein level. The same was true for N in the feces, but given the high digestibility of dietary protein, N-feces always remained below 6 g N per kg fish produced. Starting from dietary protein levels of 35% going up, part of the feed remained uneaten. With the 50% protein diet almost 32% of the total N-waste produced came from uneaten feed.

Figure 6: Final weight and N-waste production of 50-g fishes fed during 45 days at 15-g kg-0.8 d-1, diets with varying dietary protein levels. Carbohydrates and protein made up 75% of the diet on a wet weight basis.

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The simulated waste production for fish fed a 35% protein diet at increasing feeding levels is given in Figure 7. The fish reached a maximum weight of 154 g at a feeding level of 20 g kg-0.8 d-1. The lowest N-waste production of 30 g N per kg fish produced was obtained with a feeding level of 10 g kg-0.8 d-1. Below or above that level, N-waste production increased. Increasing the feeding level from 10 to 15 g kg-0.8 d-1 resulted in a 64 % increase in weight gain and a 7 % increase in N-waste production. Increasing the feeding level from 15 to 20 g kg-0.8 d-1 resulted in a 14% in weight gain and a 44% increase in N-waste production. When more food is given, it remains uneaten, resulting in no extra growth and more N-waste.

Figure 7: Final weight and N-waste production of 50-g fishes fed during 45 days a 35 % protein diet at feeding level varying between 5 and 40 g kg-0.8 d-1.

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Comparison of waste production to the amount of waste recovered

Figure 8 reviews the agreement between simulated and observed final weight of the different growth trials. The ARE was 4.9%. Except for the pond data, the simulated values are slightly higher than the observed values.

Figure 9 compares the simulated waste production in each system to the amount of waste that was discharged from the system or accumulated in the system. Assuming tilapias realize their growth only based on the administrated feed, the simulated N-waste production is given. In ponds, in total 60% of the simulated N-waste produced could be measured as accumulated in the water column and soil, and 40% could not be accounted for. In the indoor recirculation system N-lost was 51% of the total amount of simulated N-waste produced by the fish. Of the total amount of N-waste produced only 49 % could be traced in the form of N-accumulated (13%) and N-discharged in the effluent (33%). Similarly, in the outdoor recirculation system 33 and 53% of the estimated waste production by the fish could be traced back with the 12 and 24-hour discharge period, respectively. In all cases, the biggest source of N-waste was N-ammonia.

Figure 8: Agreement between observed and simulated values for fish based on data set 4.

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Figure 9: Comparison between the simulated N-waste production (sim) and the amount of N-waste that could be traced back in the systems (exp). The percentage of sim that could not be traced back is given in the N-lost bar.

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Discussion

Estimating the amount of uneaten feed

Within one size group, the multiphasic equation gives a clear prediction of the transition point where a higher protein ration level does not yield more growth. Unfortunately, this was done for 3 size groups of tilapias. Therefore, the linear relation relating maximum daily protein ration to individual body weight was determined with 3 data points only (Figure 3). For larger fish, the protein rations decrease too fast. Using equation 2, the protein ration becomes 0 for fishes above 250 g. Through calibration, the slope of equation 2 was raised, increasing the fish size where protein ration becomes 0 to 295 gram. With more data points, especially in the larger weight ranges, an exponential function could be fitted with and asymptotic value for protein ration in large fish.

Using only data for small fishes, equation 2 clearly improved the agreement between observed and simulated values, indicating that uneaten feed is a problem. Even when fishes are observed ingesting the food, still a part can be wasted with the water current over the gills during mastication. Van der Meer et al. (1997) found that Colossoma macropomum fed ad libitum spilled nearly 30% of the feed apparently consumed. When the feeding level was reduced to 60% of the ad libitum ration the amount of wasted feed became negligible.

Many factors besides protein feeding level and fish size influence feed intake. Van Dam and Pauly (1995) based feed intake on O2 availability. This approach is strongly related to the ability of the fish to take up O2 (gill surface, oxygen pressure in water column). Considering food availability and food composition this approach allows estimating the energy available for metabolism and consequently growth. A condition for the use of this approach is the availability of data of O2 concentration over 24-hour periods. This information is often not available for outdoor systems. Only with indoor systems that aerate constantly, O2 levels remain constant during the day. Models exist to predict algae production in ponds (Jiménez-Montealegre et al. 1995) which can be adapted to predict fluctuations in O2 levels. If these models could be linked, the lack of data of 24-hour O2 profiles could be avoided.

Estimating of N-waste production

Nijhof (1994) calculated the effect of feed composition, feed conversion and uneaten feed on waste discharge in an eel recirculation system, using a time step of 1 day. Although interesting, this information cannot be used for design purposes. Figure 6 and 7 give insight on the effect of feed composition and feeding level on waste production in relation to production. When the fish density and volume of a system are known, the total waste load can be calculated. The model works with a time step of integration of 3 hours, and changes in nutrient loading within a 24-hour period are also calculated. Such information is useful to design water flow, biofilter size and shape, and the sedimentation unit. In addition, the model allows to select feeding strategies that lead to a minimal waste production. The approach can also be extended to other types of waste production, like the amounts of organic matter produced, CO2 production, COD load, etc., and be used as a tool for the development of low pollution diets (New 1996)

Comparison of waste production to the amount of waste recovered

The highest fraction of the simulated waste production was recovered in ponds, mainly in the sediment. Ammonia present in the water column is taken up by algae, of which a large fraction accumulates at the bottom (Brune and Drapcho 1991). This might explain in part why in ponds a higher percentage of the N-waste produced could be traced back than in indoor recirculation systems where no N-waste is stored in life or dead algae biomass. Algae were also present in outdoor recirculation systems, but given the small water volume and surface area the contribution of algae to N-uptake or storage is negligible.

In all field experiments, including the experiment in Wageningen, feed formulation was based on ingredients commonly included in commercial tilapia feeds in Puerto Rico. These pellets had the disadvantage that they felt apart within seconds upon contact with water, resulting in a higher than normal level of feed spillage. The calibration and validation of the model was done based on feeding trials in which water-stable sinking pellets were used. This might explain why for the recirculation systems the simulated weights were slightly higher than the observed weights. The situation in ponds was different, as besides access to dietary N, tilapias can also feed on natural feeds. Research by Schroeder (1983) and Anderson (1987) indicated that in ponds receiving commercial pellets, natural feeds contributed 50-80% to the fish or shrimp production. More research is needed, however, if correct, this model should not be applied to ponds, where fish have easy access to detritus, algae, biofilms (including periphyton) and benthic organisms as alternative food sources. The fact that growth was simulated well should then be regarded as a matter of luck.

In recirculation systems, an N recovery level below 50% has been recorded for rainbow trout (van Weerd et al. 1995) when considering total N-input. In this study it was assumed that no N got trapped in the biofilms present in the recirculation systems, as these were already fully developed at the start of each study. It should be checked whether this assumption was correct. Which processes cause the high levels of N-lost needs further investigation. Some of the N-loss might be explained through volatilization of gaseous N (Avnimelech et al. 1992).

Conclusions

The model simulated well growth and fat deposition in tilapia when applying feeds of varying composition at different daily feeding rations. In situations where fish has limited access to natural foods, the model can be used to predict N-waste production, including the amount of N in uneaten feed. Also other types of metabolic wastes can be quantified with the model. However, the model does not work well for fishes weighing above 250 gram. Data on feed utilization by tilapias larger than 250 gram are needed to adjust the relationship between maximum protein intake and body weight. As long as the feeding ecology in ponds is not well understood, use of the model for pond situations might lead to erroneous conclusions.

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