Plantpath.ifas.ufl.edu



CURRICULUM VITAE

August 2020

Chenggang Wang

Address: Department of Plant Pathology

University of Florida

1453 FIFIELD HALL, 2550 HULL ROAD

Gainesville, FL 32611

Phone: (352) 888-2959 Gender: Male

Date of Birth: May 10, 1974 Email: chenggangwang@ufl.edu

• Screening and identification of plant host genes involved in the disease resistance process.

• Screening and identification of pathogenic factors of fungal pathogens.

• Plant-pathogen interaction.

• 2019 ~ Research scientist, Department of Plant Pathology at University of Florida

• 2018 ~ 2019 Biological scientist III, Department of Plant Pathology at University of Florida.

• 2017 ~ 2018 Biological scientist III, Department of Microbiology and Cell Science at

University of Florida.

• 2011 ~ 2017 PostDoc, Department of Microbiology and Cell Science at University of Florida.

• 1997 ~2004 Teacher, Duanji Middle School, Henan, China.

• 2007 ~ 2011 PhD Stage, Major in Biochemistry and molecular biology.

• Chinese Academy of Agricultural Sciences, China.

• 2004 ~ 2007 Master Stage, Major in Plant genetics. Hubei University,China.

• 1993 ~ 1995 Undergraduate Stage, Major in Bioscience. Xinyang Normal University, China.

• 1.Chenggang Wang, Xiaoen Huang, Qi Li, Yanping Zhang, Jianliang Li, Zhonglin Mou Extracellular pyridine nucleotides trigger plant systemic immunity through a lectin receptor kinase/BAK1 complex. Nature communications, 2019, 10 (1), 1-16.

• 2.Chenggang Wang, Xudong Zhang, JianLiang Li, Yanping Zhang, and Zhonglin Mou The Elongator Complex-Associated Protein DRL1 Plays a Positive Role in Immune Responses against Necrotrophic Fungal Pathogens in Arabidopsis. Molecular Plant Pathology, 2018, 19 (2), 286-299.

• 3.Chenggang Wang, Mingqi Zhou, Xudong Zhang, Jin Yao, Zhonglin Mou The Arabidopsis LecRK-I.8 Is an Extracellular Nicotinamide Adenine Dinucleotide Receptor.   eLife 2017; 6:e25474  DOI: 10.7554/eLife.25474.

• 4.Chuanfu An*, Chenggang Wang*, Zhonglin Mou The Arabidopsis Elongator complex is required for nonhost resistance against the bacterial pathogens Xanthomonas citri subsp. citri and Pseudomonas syringae pv. phaseolicola NPS3121. New Phytologist, 2017,  214 (3), 1245-1259.

• 5.Chenggang Wang, Xuezhu Du and Zhonglin Mou The Mediator Complex Subunits MED14, MED15, and MED16 Are Involved in Defense Signaling Crosstalk in Arabidopsis. Frontiers in Plant Science, 2016.

• 6.Chenggang Wang, Xudong Zhang, and Zhonglin Mou Comparison of nicotinamide adenine dinucleotide phosphate-induced immune responses against biotrophic and necrotrophic pathogens in Arabidopsis thaliana. Plant Signaling & Behavior, 2016,11(6).

• 7.Chuanfu An, Yezhang Ding, Xudong Zhang, Chenggang Wang, Zhonglin Mou Elongator Plays a Positive Role in Exogenous NAD-Induced Defense Responses in Arabidopsis. Molecular Plant-Microbe Interactions, 2016, 29 (5), 396-404.

• 8.Chenggang Wang, Jin Yao, Xuezhu Du, Yanping Zhang, Yijun Sun, Jeffrey Rollins, Zhonglin Mou. The Arabidopsis Mediator Complex Subunit16 Is a Key Component of Basal Resistance Against the Necrotrophic Fungal Pathogen Sclerotinia sclerotiorum. Plant Physiology, 2015, 169 (1), 856-872.

• 9.Chenggang Wang, Yezhang Ding, Jin Yao, Yanping Zhang, Yijun Sun, Zhonglin Mou. Arabidopsis Elongator subunit 2 positively contributes to resistance to the necrotrophic fungal pathogens Botrytis cinerea and Alternaria brassicicola. The Plant Journal, 2015, 83 (6), 1019-1033.

• 10.Xudong Zhang, Chenggang Wang, Yanping Zhang, Yijun Sun, Zhonglin Mou. The Arabidopsis mediator complex subunit16 positively regulates salicylate-mediated systemic acquired resistance and jasmonate/ethylene induced defense pathways. The Plant Cell, 2012, 12;24(10):4294-309.

• 11.Chenggang Wang, Dan Li, Liming Xu, Caihua Dong, Junyan Hang, Juping Fang, Shengyi Liu. Over-expressionof BnERF50 from Brassica napus enhanced transgenic Arabidopsis resistance to Sclerotinia sclerotiorum. Chinese Journal of Oilcrop Science, 2011, 33 (5): 427-432.

• 12.Chenggang Wang, Li Cai, Caihua Dong, Junyan Huang, Meixia Zhao, Rong Zhou, Shengyi Liu Over-expression of BnERF104 from Brassica napus enhanced transgenic Arabidopsis resistance to Sclerotinia sclerotioru. Chinese Journal of Oilcrop Science, 2011, 33 (4): 325-330.

• 13.Biao Zhang, Junyan Huang, Shengyi Liu, Meixia Zhao, Chenggang Wang, Caihua Dong. Establishment of a Rapid Identification Method to Determine Differences in Susceptibility of Cercospora Sojina Hara by Hyphae Inoculated on the Excised Leaf Surface. Journal of Anhui Agricultural Sciences, 2011 , 39 (21) : 12714-12718.

• 14.Guohua Chai, Zetao Bai, Fang Wei, Graham J. King, Chenggang Wang, Lei Shi, Caihua Dong, Hong Chen, Shengyi Liu Brassica GLABRA2 genes: analysis of function related to seed oil content and development of functional markers. Theor Appl Genet, 2010,120:1597–1610.

• 15.Bai Zetao, Chai Guohua, Shi Lei, Xu Liming, Chengang Wang, Guo Xuelan, Dong Caihua, Liu Shengyi Isolation and Functional Characterization of GLA BRA 2 Promoter Related to Trichome Development in Brassica napus. Journal of Agricultural Biotechnology, 2010, 18(2), 210-217.

• 16.Wenyan Wang, Chenggang Wang, Bang-Lian Huang, Bangquan Huang. Agrobacterium tumefaciens-mediated transformation of Lesquerella fendleri L., a potential new oil crop with rich lesquerolic acid. Plant Cell Tiss Organ Cult, 2008, 92:165–171.

• 17.Chenggang Wang, Changchun Li, Yu Xie, Youqi Liu, Bangquan Huang. Cloning and hpRNA vector construction for Brassica napus glycolate oxidase gene (GO). Journal of Hubei University, 2007, 29(3):286-324.

• 18.Chenggang Wang; Wang Wenyan; Xie Yu; Huang Bangquan Cloning and hpRNA Vector Construction for fad2 Genes in Brassica napus and Lesquerella fendleri. Journal of Hubei University, 2007, 29(1):89-92.

• 19.Chenggang Wang, Juhong Zhang, Yu Xie, Youqi Liu, Bangquan Huang. Cloning and hpRNAi Vector Construction of Brassica napus Pyrophosphate-dependent Phosphofructokinase (PFK). Molecular Plant Breeding, 2006, 4(2):199-204.

• 20.Chenggang Wang, Yu Xie, Changchun Li , Youqi Liu , Bangquan Huang. Cloning and hpRNA vector construction for genes of cruciferin, napin, oleosin and PEPC in Brassica napus. Chinese journal of oil crop sciences, 2006, 28(3):245-250.

• 21.Youqi Liu; Juhong Zhang; Chenggang Wang; Bangquan Huang Cytoembryological and Morphological Characterization of a Polyembryonic Mutant Brassica napus twn-119. Journal of Hubei University, 2006, 28(2):187-191.

• 22.Juhong Zhang; Yu Xie; Chenggang Wang; Bangquan Huang Application of Orthogonal Design in Tissue Culture of Isatis tinctoria. Journal of Hubei University, 2006, 28(2):183-186.

Served as editor for two Journal (Frontier in Plant Science; Journal of Plant Pathology & Microbiology)

Reviewed 63 papers for 15 Journal (Journal of Experimental Botany, the Plant Cell Report; Euphytica, Australasian Plant Pathology, BMC Plant Biology, Frontier in Microbiology, Scientific Report, Plant Physiology and Biochemistry et al.)

• Patent: CN 201010230380. Issued in October, 2010.

Shengyi Liu; Yueying Liu; Chenggang Wang; Junyan Huang; Caihua Dong. Brassica napus disease-resistance related gene BnERF56 and application.

• Patent: CN 201110024607. Issued in Januanry, 2011.

Shengyi Liu; Yueying Liu; Rong Zhou; Chenggang Wang; Caihua Dong; Junyan Huang. Brassica napus resistance-related gene BnWRERF50 and preparation method as well as application.

2018~ Department of Plant Pathology at University of Florida With Dr.Jeff Rollins

• Developing the simple, efficient and low-cost CRISPR-Cas9-mediated gene editing in

Sclerotinia sclerotiorum by comparing different CRISPR systems: CRISPR/Cas9

ribonucleoprotein complexes and DNA plasmid (pCas9:U6-gRNA and pCas9:TrpC-

gRNA) that expresses both Cas9 and gRNA.

• Screening and identification of effectors in Sclerotinia sclerotiorum using bioinformatics

tools and transcriptome data in combination with biochemical and genetic methods.

2011~2018 Department of Microbiology and Cell Science, University of Florida with Dr.

Zhonglin Mou

• Identification of Extracellular nicotinamide adenine dinucleotide (NAD) receptor in

Arabidopsis through genetic screen for mutants with altered NAD induced resistance

against Pseudomonas syringae pv. maculicola ES4326.

• The Arabidopsis Mediator Complex Subunit16 Is a Key Regulator of Basal Resistance

Against the Necrotrophic Fungal Pathogen Sclerotinia sclerotiorum.

• The arabidopsis elongator subunit 2 positively contributes to resistance to the

necrotrophic fungal pathogens Botrytis cinerea and Alternaria brassicicola.

• The Deformed Roots and Leaves 1 positively regulates plant Immunity to the

necrotrophic fungal pathogens through its ATP/GTP and calmodulin binding activity.

2007~2011 Chinese Academy of Agricultural Sciences with Dr. Shengyi Liu

• Over-expressionof BnERF50 from Brassica napus enhanced transgenic Arabidopsis

resistance to Sclerotinia sclerotiorum.

• Over expression of BnERF104 from Brassica napus enhanced transgenic Arabidopsis

resistance to Sclerotinia sclerotiorum.

• The AP2/ERF domain transcription factor ERF104 confers resistance to Sclerotinia

sclerotiorum via salicylate and ethylene signaling pathways in Arabidopsis.

• Brassica GLABRA2 genes: analysis of function related to seed oil content and

development of functional markers.

2004~2007 Hubei University with Dr. Bangquan Huan

• Cloning and hpRNA Vector Construction for fad2 Genes in Brassica napus and Lesquerella fendleri.

• Cloning and hpRNA vector construction for Brassica napus glycolate oxidase gene (GO)

• Cloning and hpRNAi Vector Construction of Brassica napus Pyrophosphate-dependent Phosphofructokinase (PFK)

• Cloning and hpRNA vector construction for genes of cruciferin, napin, oleosin and PEPC in Brassica napus.

• Agrobacterium tumefaciens-mediated transformation of Lesquerella fendleri L., a potential new oil crop with rich lesquerolic acid.

Genetics and Molecular Biology of Plants

• General molecular technologies: DNA recombination technology, cDNA library construction, DNA, RNA and protein extraction, site-directed mutagenesis.

• Gene expression analysis: northern blotting, real time PCR, RT-PCR, reporter gene assay (whole-mount GUS staining, subcellular localization of GUS- and GFP- tagged proteins, etc.), northern blotting, southern blotting, western blotting, in situ hybridization.

• Genetic analysis of mutants and functional analysis of genes: genotyping, positional cloning of genes, analysis of mutant phenotypes, gene overexpression, recombinant protein expression and purification, RNA interference (RNAi).

• Epigenetics research: analysis of DNA methylation and siRNA by next-generation sequencing.

Cell Biology and Biochemistry

• Light microscopy; Fluorescence microscopy; Confocal microscopy.

• Tissue culture and plant regeneratio; Agrobacterium-mediated genetic transformation of B.napus cotyledon and hypocotyls explants.

• GUS staining; Iodine stain for starch; Trypan blue stain for cell death; diaminobenzidine stain for hydrogen peroxide.

• In vitro pull-down assays; Yeast-two-hybrid; Bimolecular fluorescence complementation (BiFC); Co-Immunoprecipitation (Co-IP); Chromatin immunoprecipitation (ChIP).

Genomics and Bioinformatics

• Sequence assembly and sequence mapping.

• Database similarity searches, sequence alignment, comparative sequence analysis.

• Motif finding and protein functional analysis (MEME/MAST, BLOCKS, PHI-Blast, Pfam, PROSITE etc).

• Molecular phylogenetic analysis (PAML, Phylip, MEGA etc).

• American Society of Plant Biologists (ASPB).

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