Laboratory 9: Plasmid Isolation

In this lab, you will lyse the cells with base combined with the anionic detergent sodium dodecyl sulfate (SDS). This combination has been used for more than 20 years for plasmid isolation. Exposure of bacterial suspensions to the strongly anionic detergent at high pH opens the cell wall, denatures chromosomal DNA and proteins, and releases ... ................
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