APPLICATION FOR NATIONAL FORAGE LEGUME VARIETY …



SAVE this form to your desktop or computer. Enter required information and upon completion, return to nvrb@ by clicking on this link and attaching the application.ASSOCIATION OF OFFICIAL SEED CERTIFYING AGENCIESALFALFA & MISCELLANEOUS LEGUME VARIETY REVIEW BOARDWHITE CLOVER APPLICATION – PART B – 2021This application – Part B – must be submitted along with Part A(please remember, you only need to submit ONE Part A application for multiple Part B applications)Please email the completed applications to: nvrb@All information provided on this application shall be maintained in complete confidence by the Association of Official Seed Certifying Agencies (AOSCA), its staff, and individual members of the AOSCA Variety Review Board. Each member of the Review Board will be required to sign a statement to this effect prior to their receipt of any applications for review. Upon completion of the review process, reviewers will be required to destroy or delete all applications in their possession. One copy of each application will be maintained on file in the AOSCA office.APPLICATION FOR REVIEW OF WHITE CLOVER (Trifolium repens) VARIETIESFOR CERTIFICATIONApplicant DateVariety NameExperimental DesignationIT IS THE APPLICANT’S RESPONSIBILITY TO SUBMIT AN AMENDMENT APPLICATION FOR NAME CHANGE WHEN THE PERMANENT VARIETY NAME HAS BEEN SELECTED.I. Origin, breeding history, and varietal maintenanceA. Provide a statement of origin, breeding procedures or methods, and selection criteria used in developing the variety. The statement should include the following information:Breeding methodGermplasm sources and percentagesSelection criteria – specific traits. Indicate race/strain of pests used in selectionNumber of clones and plants comprising a synthetic variety (if applicable)Breeder seed productionHow seed was bulked (e.g., equal weight of seed from each clone or other procedure).Synthetic generation of breeder seed (e.g., Syn 1, Syn 2, etc.).Location and year of first breeder seed production.B. Seed classes to be used, limitations on age of stand, and areas of production for each class.Seed ClassProductionSyntheticGenerationLength of StandAllowedLimitation on Areasfor Seed ProductionBreederFoundationRegisteredCertifiedOnly the synthetic generations given for the above seed classes are recognized as representing this variety. No supporting evidence should be used in this application from synthetic generations other than those for the Breeder, Foundation, Registered, and Certified classes listed here.C. Describe procedures for maintaining breeder and foundation seedstock. State the party to be responsible for maintaining seedstock.State any additional requirements or limitations necessary to maintain varietal characteristics.II. Testing and adaptationA. List states and areas within states where tested for forage yield and/or persistence Present data from each location in III. A.).List:Areas of adaptation, which must be supported by test locations and data. 1 = North Central 2 = East Central 3= Southeast4 = Southwest 5 = Moderately Winterhardy Intermountain6 = Winterhardy Intermountain7 = Great Plains8= Other (Specify) __________________________Areas of intended culture.III.Provide evidence of agronomic performance, including data on yield (in T/A) and persistence. Data may be from tests conducted by private firms, Agricultural Experiment Stations, or USDA.Forage yield data is required from at least six location-years from at least two distinct geographic locations. Two locations must be at least 100 miles apart. If the variety is designated for a limited area of use, the 100-mile requirement may be relaxed. However, strong justification and documentation must accompany such a request. Seeding year forage yield data cannot be used to satisfy this requirement. One location must have at least two harvest years beyond seeding year if the application is emphasizing persistence. If persistence is being emphasized then first year data is acceptable and data from two or more trials must be included in the six location-year requirement. Each harvest year must be listed separately.Summarize forage yield data below: ----------------------Total Yield (DM T/A) ----------------------Test LocationDate Planted Mo/YrSynGenYearHarvestedNo.CutsThis VarietyCheck 1(Insertname)Check 2 (Insertname)LSD.05CV%--------------------Mean Annual Yield (DM T/A) --------------------# of YearsHarvestedTotal #of HarvestsThisVarietyCheck 1(Insert name)Check 2(Insert name)Ck 1 ComparisonCk 2 ComparisonB.Persistence.Enter dates of both initial and final stand estimates. Data must come from the area of adaptation and from stands at least 24 months old. Comparison data are needed on two (2) check varieties.--------------------% Stand-------------------- ---Check Varieties---Test LocationSynGenDateSeededMo/YrNo.of YearsHarvestedNo. ofHarvestsDate ofReadings(Mo/Yr)Initial/FinalThis VarietyInitial/Final(name)(name)LSD.05CV%Initial/FinalInitial/FinalIV. PEST RESISTANCENo minimum number of pest evaluations is required. Adequate documentation must accompany claims of pest resistance. Provide reference to materials and methods used for each pest tolerance determination. Suggested procedures are cited below. A format for data on pest reaction is provided. Copy and paste this format for multiple pest reactions.PLEASE FOLLOW THESE INSTRUCTIONS CAREFULLY WHEN REPORTING PEST RESISTANCE RESULTS.When claiming specific reaction of your variety to insects, diseases, and/or nematodes, you must furnish comparative data on the reaction to the pest using accepted procedures as cited below (following application requirement 10) for those pests or provide documentation of procedures used for those pests not listed. Each disease, insect, or nematode test must include designated resistant and susceptible checks using check standards when they have been indicated. Data may be from tests conducted by private entities, Agricultural Experiment Stations, or the USDA. Tests using unadjusted data showing a resistant or susceptible check falling outside of acceptable ranges will not be accepted. Statistics must include the test mean (mean of all entries in test), LSD (.05), and CV (%) for unadjusted % resistance and DSI data that are reported. Resistance levels should be characterized using % resistant plants as follows: S = <6%, LR = 6-14%, MR = 15-30%, R = 31-50%, HR = >50%. NOTE: If a pest reaction of the variety falls on or just above an adjusted resistance category level * (+2% for LR, MR, and R; +3% for HR) and the higher rating is claimed, results of a second test must be reported. If the two tests do not agree, the lower rating is appropriate unless further testing supports the higher rating. * (Two agreeing tests are needed for: LR = 6% and 7%; MR = 15% and 16%; R = 31% and 32%; andHR = 51%, 52%, and 53%)Format for data on pest reaction (copy and paste for multiple pests)Pest: causal organism:Common name:Test conducted by:at:Test conducted in: field:Laboratory:Greenhouse:VarietyResistanceClassYearTestedSynGenAverageResponse*Number ofPlants EvaluatedTest Variety1.Check name (insert)Resistant2.Check name (insert)Susceptible3.Test Mean:L.S.D. (.05%)C.V.* Indicate average response of varieties as prescribed by the procedure, such as Disease Severity Index, Percent Survival, etc.Suggested Procedures to Evaluate the Following Pests:Northern Anthracnose (Aureobasidium caulivor) Reference: Smith and Maxwell. 1973. Crop Sci. 13:271-273. DSI classes 1 and 2 considered as resistant.Target Spot (Stemphylium sarcinaeforms) Reference: Murray, Maxwell and Smith. 1976. Plant Dis. Rept. 60: 35-37. DSI classes 1 and 2 considered as resistant.Sclerotinia Rot (Sclerotinia trifoliorum) Reference: Marum, Smith and Grau. 1994. Euphytica 77:257-261. DSI classes 1 and 2 considered as resistant.Aphanomyces (Aphanomyces euteiches) Reference: Tofte, Smith, and Grau. 1991. Crop Sci. 31:1141-1144. DSI classes 1 and 2 considered as resistant.Fusarium Wilt (Fusarium oxysporum) Reference: Veruto, Smith and Grau. 1995. Plant Dis. 79:406-410. DSI classes 1 and 2 considered as resistant.Root knot Nematode (Meloidogyne arenaria, M. hapla, M. incognit, and, M. javanica) Reference: Quesenberry, Baltensperger, and Dunn. 1986. Crop Sci. 26:61-64. DSI class 0, 1, and 2 considered as resistant.Root-lesion Nematode (Pratylenchus penetrans) Reference: Kimpinski, Christie, and Choo. 1992. Forage Notes 36:33-35.Stem Nematode (Ditylenchus dipsaci) Reference: Whitehead, A.G. 1992. Ann. Appl. Biol. 120:73-81Yellow Clover Aphid (Therioaphis trifolii) Reference: Gorz, Manglitz, and Haskins. 1975. Crop Sci. 19:257-260.Pea Aphid (Acyrthosiphon pisum) Reference: Gorz, Manglitz, and Haskins. 1975. Crop Sci. 19:257-260.Lesser Clover Leaf Weevil (Hypera nigrirostis) Reference: Gorz, Manglitz, and Haskins. 1975. Crop Sci. 15:279-280.V. VARIETY OBJECTIVE DESCRIPTION1. Classifications:a.Small (T repens L. f. repens)b.Intermediate (T. repens f. hollandicum Erith ex Jav. & Soo)c.Large (T. repens var. giganteum)2. Maturity:a.Percentage plants flowering in seedling year:b.Time of flowering (50% of plants in bloom after January 1 from spring growth in non-seeding year):c.Days earlier than standard variety:(name)d.Days later than standard variety:(name)3. Productive persistence (Usual duration of productive stand)a.Annual:b.Biennial:c.Perennial (3-4 years - show evidence in Section III. A.)4. Flower color (determined on a minimum of 100 flowering plants) based on Munsell Color System values or ranges for colors other than white:a.% Whiteb.% Pinkishc.% Pink or darker5. Leaf marking (at 50% flowering)a. Presence of mark: Of a total of 100 flowering plants, give percentage marked and unmarked (Total = 100%)% marked% unmarked6.Stolon density reported as “number of stolons intersecting at within 1 meter” 7. Variants: Describe variants observed, including frequency of occurrence. Do not include off-types.8. List any other traits or special markers that may be useful for identifying the variety, including characteristics determined by use of biochemical methods. Please attach a one-page description/summary of your variety as you wish it published by AOSCA. This description must stand on its own. Use complete sentences, and follow the format given below, inserting your statements after the headings presented in the template on the next page. PLEASE DELETE THIS INSTRUCTION PAGE BEFORE SUBMITTING THE COMPLETED APPLICATION.White CloverVariety NameExperimental Designation Name(s)Origin and Breeding History:Prepare a statement of origin describing breeding procedures or methods and selection criteria used in developing the variety. Confidential business information is not required. The statement should include the following:The kind and name of the variety, its experimental designations, and the identity of the developer.The breeding method(s) employed in the variety’s development.Selection criteria including specific traits. Indicate race/strain of pests used in selection.The year that breeder seed was first produced.Areas of Probable Adaptation:State the probable geographic area of adaptation of this variety. Report the states where the variety has been tested for yield and proposed areas of intended use. Report states where the variety has been tested for yield and proposed areas of intended use.Agronomic and botanical Characteristics:Describe the variety in terms of winter hardiness/persistence, flower color, any morphological or physiological characteristics, and variants that may be used as identifying traits. Transfer responses given in varietal objective description to the appropriate cells in the template provided.Prepare a statement covering the variety’s reaction to pests as reported in this application. Races and species should be indicated, if known.Procedures for Maintaining Seed Stock:Describe procedures for maintaining seed stock, seed classes to be used, limitations on age of stand and generations that may be certified, other requirements or limitations necessary to maintain varietal characteristics, and the entity that will be responsible for maintaining seed stocks of the variety. State the year in which breeder seed was first produced.Certified Seed Availability and Publication of Certified Seed ProductionState when certified seed may first be offered for sale if this variety is recommended by official certifying agencies.State whether certified seed production acreage may be published by AOSCA and member agencies.PVP Information:State whether application will be submitted for protection under the Plant Variety Protection Act, and if so, whether or not the Title V certification option will be requested (to be sold by variety name only as a class of certified seed).As a means of added varietal protection, state whether or not AOSCA may provide descriptive information about this variety to the PVP office.White CloverInsert Variety Name here Insert Experimental Designation Name(s) here (Exp)Origin and Breeding HistoryAreas of Probable AdaptationAgronomic and Botanical CharacteristicsClassificationPersistence % Flowering Seeding YearLeaf Marking at 50% Flower:% MarkedFlower Color% White% Pinkish% Pink or DarkerStolon DensityNumber of stolons intersecting within one meter Description of variantsAdditional Description and/or Information about Physiology, Pest Reaction, and Other Varietal AttributesProcedures for Maintaining Seed StockCertified Seed Availability and Publication of Certified Seed ProductionGenerations Allowed –Length of Stand Limitation – Mark All That ApplyIf None, Please StateFoundationRegisteredCertifiedFoundationRegisteredCertifiedPVP Information ................
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