Introduction to Biotechnology Workshop for High School ...



Introduction to Biotechnology Workshop for High School TeachersWorkshop DatesJune 26th to June 28th Workshop SiteAnderson High School Austin, Texas, 8403 Mesa Drive 78705Laboratory used includes all equipment needed to run wet labs. This room is used to teach Biotechnology as an articulated/dual credit course throughout the school year and is fully equipped and in compliance with ACC Science safety regulations to accommodate up to 20 participants for performing wet labs. The room also has a class set of netbook computers, access to wireless printing, media station, projector and an innovation media station for presentations. Purpose: To host and conduct professional development workshops in biotechnology for high school educators to:? Learn about the tools, foundational science, and applications of biotechnology;? Encourage student interest in relevant science, technology, and careers;? Support the new CTE Texas Essential Knowledge and Skills for Advanced Biotechnology in alignment with ACC Articulated Biology 1414, Introduction to BiotechnologyWorkshop Participant BenefitsProfessional Development Certificate by ACC Biotechnology ProgramAccess to literature, curriculum and media materials from Texas Education Agency and ACC Biotechnology High School Outreach ProgramA CD or flash drive of materials presented at the workshopWorkshop ObjectivesWorkshop participants should learn:The fundamental scientific concepts underlying biotechnology and relevant laboratory techniques Effective methods for teaching these scientific concepts and laboratory techniques in the classroomEffective strategies for integrating workshop materials into participants coursesHands-on laboratory activities that illustrate concepts (if applicable)Applications of the techniques and technologies related to the chosen topic (if appropriate)Societal issues raised by the technologies (if applicable)Workshop Instructional Team: Angela ACC Biotechnology Faculty: Lecture, lead discussion and wet labsJennifer Lazare, High School Master Biotechnology Teacher, ACC Adjunct Faculty: assist with wet labs, demonstrate activities, and provide pedagogy and practical information on how to implement biotechnology in the classroomWorkshop Content: Based on Texas Education Agency Biotechnology Teacher Training Materials and ACC Introduction to Biotechnology Course. Instructors will blend lecture/discussions with hands-on activities (wet and dry labs and pedagogically useful web sites).TopicsDNA structure and functionIntroduction to gene expression and gene regulationProtein structure and function and their relationship to specific phenotypes/traitsRestriction enzymes, their role in recombinant DNA technology, and restriction analysis of DNATransformation of E. coliMolecular biology of gene cloningCurrent commercial applications of biotechnologySocietal issues related to applications of biotechnology, including one or more of the following: genetically engineered plants, stem cell research and ramifications of the Human Genome ProjectBiotechnology careers and their education requirements.Basic Biotechnology Wet Lab Skills:Proper use, maintenance and troubleshooting of biotechnology tools/equipment: micropipette, electrophoresis apparatus, thermocyclerSafely growing, storing and disposing of E. coli in the lab; proper handling of cultures andMaking solutions and media needed for classroom experimentsPreparing, handling, and staining agarose gelsClassroom preparation for wet labsAdvanced Biotechnology Wet Lab Skills: Protein Purification, Bioinformatics, Student CapstonesSchedule of EventsDay 1Biotech BasicsBackgroundLecture/DiscussionsDemo/Student Activities Available for practice or samplingWet LabLab Materials8:30-10:00What is Biotechnology? Survey of IndustryBiotech TimelineMovie Maker ProjectCurrent EventsDNA Extraction ad PrecipitationGenes in a Bottle166-2300EDU10:15-11:00Using the Micro pipetteMath Skills ExcelPipetting11:15-12:15Solutions and DilutionsSetting up a Scientific NotebookSolutions and DilutionsCalcium Chloride and Magnesium Sulfate12:15-1:00LUNCH1:00-1:30ElectrophoresisVirtual Electrophoresis LabsCasting an agarose gel1:30-2:30Mapping the Human GenomeDye Electrophoresis2:45-4:00How to fund your Biotechnology Course/Best practices/ResourcesDay 2Biotech BasicsBackgroundLecture/DiscussionsDemo/Student Activities Available for practice or samplingWet LabLab Materials8:30-9:30Restriction EnzymesPaper Restriction GelRestriction digestion of Lambda: set up digest and incubateRestriction Digestion of Lambda 166-0002EDU Agaorse Gels9:30-10:00Medical DiagnosticsRFLP WebquestRFLP case studies: Load and run gelsEdvotek: Gene Cancer, Paternity, Cholesterol, Sickle cell, Crime Scene DNA10:00-10:15Load and run restriction digest10:15-10:45Classroom management of materials11:00-12:15Stan and interpret gels12:15-1:15LUNCH1:30-2:30TransformationpGlo transformation (grow overnight)pGLO? Bacterial Transformation Kit 166-0003EDU3:00-4:00PCRPaper PCRVirtual PCRCrime Scene PCR: set up reaction-PCR overnightCrime Scene Investigator: PCR Basics Kit166-2600EDUDay 3Biotech BasicsBackgroundLecture/DiscussionsDemo/Student Activities Available for practice or samplingWet LabLab Materials8:30-10:00PCR DiagnosticsCSI Web AdventureRun and interpret PCR10:15-11:00Biotechnology Curriculum and best practicesProject Share/TEA Resources/ACC Resources11:00-12:00ImmunoassaysELISAELISA Immno Kit166-2400EDU12:00-1:00LUNCH1:00-2:30Bioinformatics/NCBIGFP BioinformaticspGlo analysis and Bioinformatics3:00-4:00Creating a Teacher Workgroup PLCStudent Capstone Projects ................
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