MODERN METHODS in BIOCHEMISTRY
MODERN METHODS in BIOCHEMISTRY
?PROTEIN MODIFICATION ?PROTEIN CROSSLINKING ?PROTEIN STAINING ?ANTIBODY MODIFICATION ?IMMUNOPRECIPITATION ?METABOLIC LABELLING
Affinity chromatography :
? matrices ? preparation ? Coupling of Ligand ? detection of reactive groups
Affinity chromatography : Principle of method
Affinity chromatography Example
Mouse brain
Affinity Chromatography Immunoprecipitation
Gel filtration
Solubilise & homogenise
50,000 x g supernatant
eluted complexes SDS-PAGE
Affinity chromatography
matrice preparation & introduce spacer
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