Biosafety Manual - University of Florida

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2014

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Biosafety

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Manual

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Business Affairs

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Division of

Environmental Health & Safety

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BioSafety Manual

University of Florida Business Affairs

Environmental Health and Safety

Box 112190 Gainesville, Florida 32611

(352) 392-1591 phone (352) 392-3647 fax

Email: BSO@ehs.ufl.edu

2014

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Emergency Contacts

General

Police Emergency: University Police Department Fire Emergency: Gainesville Fire Rescue Medical Emergency: Gainesville Fire Rescue Gas leak: Gainesville Fire Rescue

392-1111 911 911 911

Spills/Releases/Accidents: Notify the Principal Investigator, Lab Director/Supervisor and as

appropriate for the following items:

Business Hours

After Hours

Asbestos

392-3392

392-1111

Biological or Recombinant Materials

392-1591

392-1111

Select Agents

392-1591

392-1111

Chemicals (laboratory)

392-1591

392-1111

Mercury

392-8400

392-1111

Pesticides

392-1904

392-1111

Radioactive Materials: Campus

392-7359

392-1111

Radioactive Materials: Health Science Center 392-1589

392-1111

Needle Stick Hotline: 1-866-477-6824 (OUCH) Notify the Principal Investigator, or Lab Director /

Supervisor and during working hours employees must call the UF Worker's Compensation Office (UFWC) at (352) 392-4940 prior to seeking treatment for work related injuries that are not immediate medical emergencies. See chapter on exposures and incidents for additional information.

Student Health Care Center Clinic, D2-49: (352) 294-5700

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Table of Contents

Authority & Responsibilities ............................................................................................. 6 Policy Statement ............................................................................................................. 6 Responsibilities ............................................................................................................... 7 Biosafety: Fundamentals and Definitions ........................................................................ 7

Biohazards................................................................................................................................................. 8 Regulatory Authority .................................................................................................................................. 8

Biohazards Risk Assessment .......................................................................................... 9

Objective.................................................................................................................................................... 9 Risk Assessments ..................................................................................................................................... 9 Human Pathogens ................................................................................................................................... 10 Animal Pathogens ................................................................................................................................... 12 Plant Pathogens and Greenhouses ........................................................................................................ 14 Select Agents .......................................................................................................................................... 15 Recombinant and Synthetic Nucleic Acids .............................................................................................. 16 Biological Toxins...................................................................................................................................... 18 Mammalian Cell Cultures ........................................................................................................................ 19 Human Blood and Other Potentially Infectious Material.......................................................................... 19 Clinical Laboratories ................................................................................................................................ 20 Viral Vectors ............................................................................................................................................ 21

Biosecurity..................................................................................................................... 29 Control of Biohazards.................................................................................................... 32

Laboratory Practices................................................................................................................................ 32 Engineering Controls ............................................................................................................................... 33 Personal Protective Equipment ............................................................................................................... 36 Administrative Controls............................................................................................................................ 37

Decontamination and Sterilization of Biologicals ........................................................... 39

Disinfectants ............................................................................................................................................ 40 Biomedical and Biological Waste ............................................................................................................ 42 Animal Carcasses.................................................................................................................................... 44 Disposal of Biological Toxins................................................................................................................... 45 Autoclave Testing and Use...................................................................................................................... 45 Equipment Decontamination ................................................................................................................... 46 Laboratory Decontamination ................................................................................................................... 47

Emergencies ................................................................................................................. 47

Personnel Responsibilities/Assignments During a Disaster.................................................................... 47 Emergency Evacuation Procedures ........................................................................................................ 48 Fire Safety ............................................................................................................................................... 49 Break-In/Security Breach......................................................................................................................... 49 Workplace Violence................................................................................................................................. 50

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Hurricane Watch...................................................................................................................................... 50 Tornadoes and Other Natural Disasters.................................................................................................. 50 Bomb Threats .......................................................................................................................................... 50 Natural/LP Gas Leak ............................................................................................................................... 51 Suspicious Package ................................................................................................................................ 51 Explosions ............................................................................................................................................... 51 Power Outage.......................................................................................................................................... 52

Exposures and Incidents ............................................................................................... 52

Exposures................................................................................................................................................ 52 Incidents: Spills and Injuries .................................................................................................................... 54

Occupational Medicine Programs ................................................................................. 58

Bloodborne Pathogen (BBP) Program .................................................................................................... 58 Animal Contact Medical Monitoring Program .......................................................................................... 59 Respiratory Protection Program .............................................................................................................. 60 BioPath Medical Monitoring Program ...................................................................................................... 61 Vaccination Policy for Research Personnel ............................................................................................ 62

Packaging, Shipping and Transport of Biological Materials........................................... 64 Permits .......................................................................................................................... 66

CDC ......................................................................................................................................................... 66 USDA....................................................................................................................................................... 66 U.S. Fish and Wildlife Service ................................................................................................................. 67 Florida Department of Agriculture and Consumer Services .................................................................... 67 Bioagent Export Control .......................................................................................................................... 67

Registration Information for Researchers...................................................................... 67

Biological Agent Registration................................................................................................................... 67 Recombinant and Synthetic Nucleic Acid Registration ........................................................................... 68 Acute Toxin Registration ......................................................................................................................... 68 Administrative and Technical Change Amendments .............................................................................. 69 Human Gene Therapy Projects ............................................................................................................... 69 Laboratory Biosafety Levels and Checklists............................................................................................ 69 New Researcher Information.....................................................................Error! Bookmark not defined. Laboratory Close-outs ...............................................................................Error! Bookmark not defined.

Special Issues ............................................................................................................... 70

HIV Research Laboratories ..................................................................................................................... 70 Non-Human Primates (monkeys) B Virus Information ............................................................................ 71 Q fever Control ........................................................................................................................................ 72 Tuberculosis Research Laboratories....................................................................................................... 73 Vaccinia and Other Pox Virus Research Laboratories ............................................................................ 74

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