Western Connecticut State University

The next step was to transform the insert containing plasmid into BL21 cells for protein expression. Isopropyl β-D-1-thiogalactopyranoside (IPTG) is necessary for protein expression. IPTG is similar to allolactose, which is needed to activate the lac operon. The difference between IPTG an allolactose is that IPTG can’t be broken down by the ... ................
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