Materials - The cDNA of NaChBac from Bacillus halodurans ...
Auto-induction of expression at 37 °C was performed in 30-l cultures of E. coli strain BL21 Star™ (DE3) (Invitrogen) cells, harbouring the plasmid pRARE2 (Novagen) and pMPSIL0101A, using LB ... ................
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