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strain BL21 (DE3) competent cells. A single colony was inoculated into 50 mL of Luria-Bertani (LB) medium containing 100 μg/mL of ampicillin and incubated overnight at 37°C. Then, the overnight culture was transferred to 2 liters of fresh LB medium for large-scale protein production by growing at 37°C. ................
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