Research Central Software Suite - Blackrock Micro

Manufacturer

630 Komas Drive | Suite 200

Salt Lake City | UT 84108 | USA

P +1 (801) 582-5533 | F +1 (801) 582-1509



Research Central

Software Suite

Instructions for Use

Revision 5.00 / LB-0574 ¨C Research Central Software Suite: Instruction for Use ¡ª 2019/02

? 2019 Blackrock Microsystems, LLC

Table of Contents

What This Manual Covers ............................................................. 6

System Description ......................................................................... 6

Minimum System Requirements ...................................................... 6

Setup............................................................................................... 6

Installing Central Software Suite ................................................... 6

Configuring an Ethernet Port ........................................................ 7

Connecting Multiple PCs to One NSP ........................................... 8

Connecting Two NSPs to One PC ................................................ 8

Central ........................................................................................... 9

Central Main Window ...................................................................... 9

Menu Bar ...................................................................................... 10

File.............................................................................................. 10

Tools........................................................................................... 11

Options ....................................................................................... 11

Windows ..................................................................................... 12

Hardware Configuration .............................................................. 13

Channel Sorting .......................................................................... 13

Configuring Channel Properties .................................................... 13

Front End Analog Inputs ............................................................. 14

Analog Output............................................................................. 15

Analog Output: Continuous Monitor ............................................ 16

Analog Output: Spike Monitor ..................................................... 16

Analog Output: Sine Waveform................................................... 17

Analog Output: Custom Waveform ............................................. 17

Analog Output: Triggering ........................................................... 18

Digital Input................................................................................. 18

Digital Output .............................................................................. 19

Revision 5.00 / LB-0574 ¨C Research Central Software Suite: Instructions for Use

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Digital Output: Monitor Electrode ................................................ 19

Digital Output: Timed .................................................................. 20

Digital Output: Triggered ............................................................. 20

Serial I/O..................................................................................... 20

Settings ......................................................................................... 21

Global Settings ........................................................................... 21

Filters.......................................................................................... 22

Auto Thresholding....................................................................... 22

Spike Sorting .............................................................................. 23

Tools Menu ................................................................................. 25

Adaptive Filtering ........................................................................ 25

N-Trode Groups .......................................................................... 26

Spike Panel ................................................................................. 27

Spike Panel Settings ..................................................................... 27

Raster Plot .................................................................................. 28

Tool Bar ........................................................................................ 28

Single Neural Channel ................................................................ 30

Channel Configuration................................................................... 30

Spike Display ................................................................................ 31

Manual Override Options .............................................................. 31

File Storage ................................................................................. 32

TOC Interface................................................................................ 32

2.x Interface .................................................................................. 33

File Storage Options ................................................................... 33

Digital Oscilloscope ..................................................................... 34

Trigger Type .................................................................................. 35

Digital Filter Editor ....................................................................... 36

Activity Map ................................................................................. 37

Revision 5.00 / LB-0574 ¨C Research Central Software Suite: Instructions for Use

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Signal-to-Noise Ratio .................................................................. 38

Neural Modulation ....................................................................... 39

Impedance Tester ....................................................................... 39

Crosstalk ..................................................................................... 41

N-Trode ....................................................................................... 42

Spike Display ................................................................................ 43

Add Comment ............................................................................. 43

nPlay Server ................................................................................ 43

How To ........................................................................................ 44

Setup Channels for Data Acquisition ............................................. 44

Sort Spike Units ............................................................................ 45

Histogram Peak Count (Automatic)............................................. 45

Hoops (Manual) .......................................................................... 45

Manual PCA ............................................................................... 46

k-means PCA ............................................................................. 47

DBSCAN PCA ............................................................................ 48

Use Adaptive Filtering ................................................................... 48

Create a Digital Filter..................................................................... 49

Rename Multiple Channels ........................................................... 49

Remotely Control Data Recording ................................................. 50

Visualize an Input Channel on the Digital Oscilloscope ................. 50

Configure Raster Plot .................................................................... 51

Change Electrode Map (Create a Map File) .................................. 52

Test Impedance with a CerePlex Headstage................................. 53

Record a Video using NeuroMotive ............................................... 54

Troubleshooting/Error Messages ................................................ 55

Support ........................................................................................ 58

Complaints .................................................................................... 58

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Table of Figures

Figure 1 ¨C Network Connections Window................................................................................... 7

Figure 2 ¨C IPv4 Properties ......................................................................................................... 8

Figure 3 ¨C Local Area Connections ............................................................................................ 8

Figure 4 ¨C Central Main Application ........................................................................................... 9

Figure 5 ¨C Central File Menu .....................................................................................................10

Figure 6 ¨C Central Options ........................................................................................................11

Figure 7 ¨C Hardware Configuration ...........................................................................................13

Figure 8 ¨C Analog Input Properties ............................................................................................14

Figure 9 ¨C Edit Multiple Labels ..................................................................................................14

Figure 10 ¨C Filter Properties ......................................................................................................15

Figure 11 ¨C Analog Output: Continuous Monitor........................................................................16

Figure 12 ¨C Analog Output: Sine Generator ..............................................................................17

Figure 13 ¨C Analog Output: Custom ..........................................................................................18

Figure 14 ¨C Digital Output Properties ........................................................................................19

Figure 15 ¨C Serial I/O ................................................................................................................20

Figure 16 ¨C Hardware Configuration Settings ............................................................................21

Figure 17 ¨C Filter Properties Display .........................................................................................22

Figure 18 ¨C Spike Sorting Properties.........................................................................................24

Figure 19 ¨C Tools Menu ............................................................................................................25

Figure 20 ¨C Adaptive Filtering ...................................................................................................25

Figure 21 ¨C N-Trode Properties.................................................................................................26

Figure 22 ¨C Spike Panel ............................................................................................................27

Figure 23 ¨C Raster Plot .............................................................................................................28

Figure 24 ¨C Single Neural Channel ...........................................................................................30

Figure 25 ¨C TOC Interface ........................................................................................................32

Figure 26 ¨C 2.x Interface ...........................................................................................................33

Figure 27 ¨C Digital Oscillloscope View ......................................................................................34

Figure 28 ¨C Trace Amplitude .....................................................................................................34

Figure 29 ¨C Digital Filter Editor ..................................................................................................36

Figure 30 ¨C Activity Map ............................................................................................................37

Figure 31 ¨C Signal to Noise Ratio ..............................................................................................38

Figure 32 ¨C Neural Modulation ..................................................................................................39

Figure 33 ¨C Auto Impedance .....................................................................................................40

Figure 34 ¨C Crosstalk ................................................................................................................41

Figure 35 ¨C N-Trode Utility ........................................................................................................42

Figure 36 ¨C nPlay Server ..........................................................................................................43

Figure 37 ¨C PCA Space ............................................................................................................45

Figure 38 ¨C Sorted Spikes .........................................................................................................45

Figure 39 ¨C PCA Space ............................................................................................................46

Figure 40 ¨C PCA Basis Waveforms ...........................................................................................47

Figure 41 ¨C Label Options .........................................................................................................49

Figure 42 ¨C Example Settings for Remote Recording ................................................................50

Figure 43 ¨C Sample CMP File ...................................................................................................52

Figure 44 ¨C Sample Spike Panel ...............................................................................................52

Figure 45 ¨C File Application Connected to NeuroMotive? ........................................................54

Revision 5.00 / LB-0574 ¨C Research Central Software Suite: Instructions for Use

? 2019 Blackrock Microsystems, LLC

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