E. Ann Steiner, MT(ASCP)SBB Blood Bank Technical Specialist Ortho ...

[Pages:44]E. Ann Steiner, MT(ASCP)SBB Blood Bank Technical Specialist Ortho Clinical Diagnostics

Why Do We Care?

Considering change? Considering automation?

To better understand current methodology, for use in troubleshooting, problem prevention

Evaluation of current alternative method(s)

Facilitate antibody identification

Serological Results That Trigger an ABID study

Certain parts of routine pretransfusion testing

Certain parts of optional steps in pretransfusion testing

The results of diagnostic, or `special', testing

Serological Results That Trigger an ABID study

Apparently `extra' reaction in ABO reverse Positive antibody screen Positive crossmatch Positive inert control, IAT control, or DAT The results of diagnostic, or `special', testing

Pretransfusion Testing

Test suspension of patient's red blood cells (RBC's) with anti-A, -B, and -D aka, the ABO/Rh forward group Test plasma/serum against known A1 and B reagent RBC's aka, the ABO reverse group Test plasma (or serum) against Group O reagent RBC's of known phenotype aka, the antibody screen or detection test

Pretransfusion Testing

Test suspension of patient's red blood cells (RBC's) with anti-A, -B, and -D aka, the ABO/Rh forward group Test plasma/serum against known A1 and B reagent RBC's aka, the ABO reverse group Test plasma (or serum) against Group O reagent RBC's of known phenotype aka, the antibody screen or detection test

Optional Serological Steps

Test suspension of patient's RBC's with: Anti-A1, -A,B, second ?D Inert control (reagent, autologous plasma)

Test plasma/serum against known A2 RBC's

Run a 3 or 4-cell screen (rather than 2-cell)

IAT autocontrol or Direct Antiglobulin Test (DAT) Perform a crossmatch

For major ABO confirmation only For the detection of IAT reactive antibodies

Optional Serological Steps

Test suspension of patient's RBC's with: Anti-A1, -A,B, second ?D Inert control (reagent, autologous plasma)

Test plasma/serum against known A2 RBC's Run a 3 or 4-cell screen (rather than 2-cell) IAT autocontrol or Direct Antiglobulin Test (DAT) Perform a crossmatch

For major ABO confirmation only For the detection of IAT reactive antibodies

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