HCG Stimulation Test - Royal United Hospital
Endocrine Dynamic Function Tests
HCG STIMULATION TEST
Indications:
Assessment of the potential of the testes to produce Testosterone e.g. in sexual ambiguity, infants with micro-penis or cryptorchidism or when the functional capacity of the testis is in question.
Contraindications:
None absolute.
Precautions:
None for this protocol. However, a prolonged HCG stimulation test can induce bone advancement and rapid growth.
Procedure:
1. Collect basal blood sample (2-3 mls, plain tube) for plasma Testosterone and
Androstenedione. A second sample (extra 2-3 mls, plain tube) should be taken, if possible, and stored frozen for further analyses as deemed appropriate. [Day 1].
2. Give HCG 2000 units intramuscularly daily for 3 days [Day 1, 2, 3].
3. Repeat blood sampling, as in step 1, 24 hours after last injection. [Day 4].
Guidelines to Interpretation:
1. Plasma Testosterone should increase at least threefold following HCG stimulation in pre-pubertal boys. The response is more marked in male infants aged 0 to 6 months and during early to mid-puberty.
2. An absent response with an exaggerated LH/FSH response to LHRH stimulation indicates primary gonadal failure or anorchia.
3. If there is a defect in Testosterone biosynthesis, there will be an increase in precursor steroid secretion following HCG stimulation.
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Please note that these guidelines apply to adults only; for children please contact Dr Amanda Billson, Consultant Paediatrician
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