Resolution of ABO Discrepancies
嚜燎esolution of ABO Discrepancies
Justin R. Rhees, M.S., MLS(ASCP)CM, SBBCM
Objectives
1. Given the results of ABO typing, correctly identify if
a discrepancy exists and if the source is most likely
in the forward or reverse type.
2. Describe in detail several causes of ABO
discrepancies due to the following:
a)
b)
c)
d)
Weak or missing reactivity in the reverse typing.
Unexpected reactivity in the reverse typing.
Weak or missing reactivity in the forward typing.
Unexpected reactivity in the forward typing.
3. Describe appropriate follow-up testing that is
necessary in the resolution of ABO discrepancies.
ABO Discrepancies
? A very important part of
pretransfusion testing
involves detection,
recognition, and
resolution of ABO
discrepancies.
? Discrepant results must
be identified and the
underlying causes
investigated.
Troubleshooting Steps
? Step 1: Repeat the test.
每 Technical errors
?
?
?
?
?
?
?
?
Specimen mix-up
Forgot to wash cells
Incorrect cell suspension
Failure to add reagents or sample
Missed hemolysis reaction (read as negative)
Didn*t follow procedure
Incorrect centrifugation
Incorrect interpretation
? Step 2: Request a new specimen.
Troubleshooting Steps
? Read the Forward Type first.
每 Note: the Forward Type reactions may not be correct
? Look at the strongest reactions.
每 The strong vs. weak reactions can provide important clues.
? Any time you encounter a discrepancy of any kind:
每 1.) Repeat the test to rule out technical errors.
每 2.) If the results are the same, record the result as:
Discrepant.
Only type O, Rh-compatible blood should be issued until the investigation is
completed.
每 3). ALL discrepancies must be investigated and resolved
before the correct ABO type can be resulted.
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