Phyto-PAM Lab
Dark adapt cells for 30 minutes in 50 mL amber bottles. Eliminate exposure to light as much as possible e.g. dim lights, close shades. Read fluorescence (Fo) Add 6 μl DCMU (resulting conc. is 10μM DCMU) Immediately read again (get stable reading) (Fm) Fv/Fm = (Fm-Fo)/Fm. Additional features of … ................
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