Determination of Biochemical Oxygen Demand (BOD5)

When volumes of sample used exceed 150 ml, additional nutrients should be added to the sample bottle. Add an additional 0.1 mL of nutrients for each 50 mL of sample used in excess of 150 mL. For example, if the sample size is 200 ml, an additional 0.2 ml is required. Completely fill two bottles with dilution water to be incubated as blanks. 6. ................
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