Preparation of competent hazard group 1 bacterial cells.



Bacterial strain

Calcium chloride

Hazardous Substances Policy - Assessment

CHEMICAL HAZARD AND RISK ASSESSMENT

School of Biosciences

|Name of |Dr S. Minchin | | |Assessment Number* |1400 |

|supervisor | | | | | |

| | Signature | | | |

|Assessor |Rita Godfrey | | |Date of Assessment |4 / 6 / 01 |

Signature

Notes A School COSHH form in Word is available on the School Server.

Available from the Health and Safety Unit.

Guidance on making an assessment is given in Making a Chemical Hazard and Risk Assessment.

Guidance is also available from Guidance on Completing the Chemical Hazard and Risk Assessment Form.

Use a continuation sheet to expand any section of this form in hard copy version.

|1 | |LOCATION OF THE WORK ACTIVITY | |Lab G10 |

| | | | | |

|2 | |PERSONS WHO MAY BE AT RISK |

| | | | |

|List names where possible |All occupants of the lab (Laura Tate, Jo Field, Dong Ling Zeng, Rita Godfrey) |

| |

| |

|3 | |ACTIVITY ASSESSED | |Preparation of competent hazard group 1 bacterial cells. |

| | | | | |

|4 | |MATERIALS INVOLVED |Attach copies of data sheet(s) | |

|NAME |AMOUNT |HAZARD |RISK PHRASES |HAZDAT NO*** |BIOSCIENCESNO*** |

| |max/stored | | | | |

|Bacterial strain | |NSH |Hazard group 1 strains only | | |

| | | | | | |

|Calcium chloride |500g |IRRITANT |R36 Irritating to eyes |1312 | |

| | | | | | |

|50mM Calcium chloride |100ml |NSH | | | |

| | | | | | |

|Freeze-thaw buffer |100ml |NSH | | | |

|(100mM CaCl2, 15% glycerol) | | | | | |

| | | | | | |

|Glycerol |2.5 litres |IRRITANT |R36 Irritating to eyes |354 | |

| | | | | | |

|LB broth |~700ml |NSH | | | |

|(10g/l tryptone | | | | | |

|5g/l yeast extract | | | | | |

|5g/l NaCl) | | | | | |

|5 | |INTENDED USE** |

| | | | |

|Give brief details and attach protocol/instructions |Preparation of competent cells for transformations covering |

|Hazard Group 1 bacterial strains only. See attached sheet. |

| |

| |

| |

|6 | |RISKS to HEALTH and SAFETY from INTENDED USE |

| | | | |

|From personal exposure or hazardous reactions. Refer to OELs, flash points, etc., as appropriate. Are pregnant women, breast-feeding mothers especially|

|at risk? |

|The only risks are from getting calcium chloride and/or glycerol in the eyes. Preparation of LB is covered |

|under assessment number1405 and so there is no risk from the broth itself. |

| |

| |

| |

|7 | |CONCLUSIONS ABOUT RISKS |

| | | | |

|Is level of risk acceptable? Can risk be prevented or reduced by change of substance/procedure? Are control measures necessary? |

|If GCP and microbiological containment level 1 rules are followed the risks to health and safety are minimal. |

| |

| |

| |

| |

|8 | |CONTROL MEASURES |

| | | | |

|Additional to Good Chemical Practice | |

|Work to containment level 1 for handling hazard group 1 organisms. |

| |

| |

| |

|9 | |INSTRUCTION/TRAINING |

| | | | |

|Specify course(s) and/or special arrangements. | |

| |

| |

| |

|10 | |MONITORING |

| | | | |

|Performance of control measures, | |

| |

| |

| |

|Personal exposure | |Health Surveillance | |

| | |

| | |

|11 | |WASTE DISPOSAL PROCEDURE |

| See School Server for Approved Procedure Document on specific Chemical Waste Disposal. |

|The supernatants contain a small amount of bacteria so this and all tubes used should be immersed in |

|Presept solution at the appropriate concentration (3.5 tablets per 10 litres) overnight before disposal. |

|Any other solutions can be poured down the sink. |

| |

| |

| |

| |

|12 | |REVIEW |

| | | | |

|Enter the date or circumstances for review of assessment (maximum review interval 5 years) | |

|June 2006 |

| |

| |

|13 | |EMERGENCY ACTION |

| | | | |

|TO CONTROL HAZARDS |To stabilize situation eg spread absorbant on liquid spill; eliminate sources of ignition, etc. |

|Mop up spills with paper towels |

| |

| |

| |

|TO PROTECT PERSONNEL |Evacuation, protection for personnel involved in clean-up, Special First Aid | |

|Protective clothing and gloves should be worn and care should be taken to avoid touching the eyes when |

|handling either CaCl2 or glycerol. |

| |

| |

| |

|TO RENDER SITE OF EMERGENCY SAFE |Clean-up/decontamination | |

|Wash site with water. |

| |

| |

| |

| |

| CONTACT PHONE |

| |

10.10.00

* Prefix T is used for Teaching Assessment Number.

** Please include amount of chemicals used and how.

*** Hazdat No is the UNICOSHH datasheet report number.

Biosciences No is the Biosciences data sheet number.

UNICOSHH IS A CHEMICAL DATABASE ON THE HEALTH AND SAFETY UNIT SERVER.

BIOSCIENCES DATA SHEETS ARE AVAILABLE IN THE SCHOOL SAFETY OFFICE.

-----------------------

414 5438

Dr S. Minchin

................
................

In order to avoid copyright disputes, this page is only a partial summary.

Google Online Preview   Download