Preparation of competent hazard group 1 bacterial cells.
Bacterial strain
Calcium chloride
Hazardous Substances Policy - Assessment
CHEMICAL HAZARD AND RISK ASSESSMENT
School of Biosciences
|Name of |Dr S. Minchin | | |Assessment Number* |1400 |
|supervisor | | | | | |
| | Signature | | | |
|Assessor |Rita Godfrey | | |Date of Assessment |4 / 6 / 01 |
Signature
Notes A School COSHH form in Word is available on the School Server.
Available from the Health and Safety Unit.
Guidance on making an assessment is given in Making a Chemical Hazard and Risk Assessment.
Guidance is also available from Guidance on Completing the Chemical Hazard and Risk Assessment Form.
Use a continuation sheet to expand any section of this form in hard copy version.
|1 | |LOCATION OF THE WORK ACTIVITY | |Lab G10 |
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|2 | |PERSONS WHO MAY BE AT RISK |
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|List names where possible |All occupants of the lab (Laura Tate, Jo Field, Dong Ling Zeng, Rita Godfrey) |
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|3 | |ACTIVITY ASSESSED | |Preparation of competent hazard group 1 bacterial cells. |
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|4 | |MATERIALS INVOLVED |Attach copies of data sheet(s) | |
|NAME |AMOUNT |HAZARD |RISK PHRASES |HAZDAT NO*** |BIOSCIENCESNO*** |
| |max/stored | | | | |
|Bacterial strain | |NSH |Hazard group 1 strains only | | |
| | | | | | |
|Calcium chloride |500g |IRRITANT |R36 Irritating to eyes |1312 | |
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|50mM Calcium chloride |100ml |NSH | | | |
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|Freeze-thaw buffer |100ml |NSH | | | |
|(100mM CaCl2, 15% glycerol) | | | | | |
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|Glycerol |2.5 litres |IRRITANT |R36 Irritating to eyes |354 | |
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|LB broth |~700ml |NSH | | | |
|(10g/l tryptone | | | | | |
|5g/l yeast extract | | | | | |
|5g/l NaCl) | | | | | |
|5 | |INTENDED USE** |
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|Give brief details and attach protocol/instructions |Preparation of competent cells for transformations covering |
|Hazard Group 1 bacterial strains only. See attached sheet. |
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|6 | |RISKS to HEALTH and SAFETY from INTENDED USE |
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|From personal exposure or hazardous reactions. Refer to OELs, flash points, etc., as appropriate. Are pregnant women, breast-feeding mothers especially|
|at risk? |
|The only risks are from getting calcium chloride and/or glycerol in the eyes. Preparation of LB is covered |
|under assessment number1405 and so there is no risk from the broth itself. |
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|7 | |CONCLUSIONS ABOUT RISKS |
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|Is level of risk acceptable? Can risk be prevented or reduced by change of substance/procedure? Are control measures necessary? |
|If GCP and microbiological containment level 1 rules are followed the risks to health and safety are minimal. |
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|8 | |CONTROL MEASURES |
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|Additional to Good Chemical Practice | |
|Work to containment level 1 for handling hazard group 1 organisms. |
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|9 | |INSTRUCTION/TRAINING |
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|Specify course(s) and/or special arrangements. | |
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|10 | |MONITORING |
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|Performance of control measures, | |
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|Personal exposure | |Health Surveillance | |
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|11 | |WASTE DISPOSAL PROCEDURE |
| See School Server for Approved Procedure Document on specific Chemical Waste Disposal. |
|The supernatants contain a small amount of bacteria so this and all tubes used should be immersed in |
|Presept solution at the appropriate concentration (3.5 tablets per 10 litres) overnight before disposal. |
|Any other solutions can be poured down the sink. |
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|12 | |REVIEW |
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|Enter the date or circumstances for review of assessment (maximum review interval 5 years) | |
|June 2006 |
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|13 | |EMERGENCY ACTION |
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|TO CONTROL HAZARDS |To stabilize situation eg spread absorbant on liquid spill; eliminate sources of ignition, etc. |
|Mop up spills with paper towels |
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|TO PROTECT PERSONNEL |Evacuation, protection for personnel involved in clean-up, Special First Aid | |
|Protective clothing and gloves should be worn and care should be taken to avoid touching the eyes when |
|handling either CaCl2 or glycerol. |
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|TO RENDER SITE OF EMERGENCY SAFE |Clean-up/decontamination | |
|Wash site with water. |
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| CONTACT PHONE |
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10.10.00
* Prefix T is used for Teaching Assessment Number.
** Please include amount of chemicals used and how.
*** Hazdat No is the UNICOSHH datasheet report number.
Biosciences No is the Biosciences data sheet number.
UNICOSHH IS A CHEMICAL DATABASE ON THE HEALTH AND SAFETY UNIT SERVER.
BIOSCIENCES DATA SHEETS ARE AVAILABLE IN THE SCHOOL SAFETY OFFICE.
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414 5438
Dr S. Minchin
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