Biol 22/ Molecular and Cellular Biology Syllabus



BIOL 22L Molecular and Cellular Biology Lab Fall 2010 Syllabus Instructors: Dr. Joanna Miller Dr. Regina Wilson Hall of Sciences 138, x3656, Hall of Sciences 145 jmiller1@drew.edu (note “1”) rwilson2@drew.eduOffice Hours: MW 10-11 am, TR 4:15-5:15 pm, W 4:15-5:15 pm, and and by appointment (email first) by appointment (email first)Course Objective: Students will be introduced to current molecular and cellular biology research procedures in a hands-on environment. Emphasis will be placed on developing good laboratory practices, including critical thinking, proper technique, record-keeping, and scientific writing. This course, along with BIOL 7 and 9, fulfills the Biology Writing in the Major [WM] general education requirement.Upon successful completion of the course, students will be able to:1. explain how to design experiments, including proper controls, 2. perform a variety of molecular and cellular biology techniques, including restriction digestion, polymerase chain reaction, western blot, and immunostaining,3. develop practical proficiency with aseptic technique when handling bacteria, yeast and cell culture cells,4. utilize online tools to manipulate nucleic acids and proteins,5. critically analyze experimental data, and6. concisely report findings in a scientific writing format including proper citations and figures. Laboratory Time and Location: 1:15 – 4:15 pm in Hall of Sciences room 133.Grading: 10% Laboratory notebook 15% Pre-lab assignments 46% Laboratory reports:12% from first,17% from second and 17% from third 24% Laboratory worksheets (2) 5 % Laboratory conduct: punctuality, courtesy, cleanliness, participation in discussionsSupplies: You must keep a bound lab notebook (a composition pad may be used if you number the pages in advance). You should also have a lab coat and safety goggles to bring to designated laboratory periods.Lab Protocols: Lab protocols will be posted on Moodle (; login with your Drew University uLogin and password) at least one day before lab. Prior to coming each lab session, it is your responsibility to print out, to read the protocol, and to secure in your notebook each page of the protocol, e.g., using tape or staples, in a manner in which the pages are easy to see and read (no folding of pages but you can remove margins).Lab Notebook: Must be a bound notebook; no spiral or loose leaf pages, no exceptions! Reserve the first page for a Table of Contents. You must complete your notebook entry as indicated in the notebook guidelines before leaving the lab in order to receive credit; your instructor or teaching assistant will initial your notebook only if it is complete. The notebook in its entirety will be graded at end of the semester; see notebook guidelines for details.Pre-lab Assignments: A downloadable (from Moodle) Pre-lab assignment is due at the very beginning of each lab period before the start of the introduction to the lab- this is incentive to come to class prepared and on time. Late students will NOT be able to turn in the Pre-lab and will receive a zero. If you know you will be late on a given day, discuss this in advance with your instructor. You may discuss the Pre-lab with others, but answers must be written independently.Lab Reports: Lab reports must be typed. Guidelines for writing reports will be posted and discussed. Hard copies of the lab reports are due on time at the *beginning* of the lab period. A grade penalty will be assessed on late assignments and NO reports will be accepted after they are one week late. You may discuss results and conclusions with others, but the entire report must be written independently.Lab Worksheets: Worksheets must be typed. Hard copies of the lab worksheets are due on time at the *beginning* of the lab period. A grade penalty will be assessed for a late lab worksheet and NO assignments will be accepted after they are one week late. You may discuss the worksheets with others, but they must be written independently.Attendance Policy: Attendance at ALL laboratories is mandatory as many laboratory exercises and skills build upon each other. Moreover, you will often be working in groups of two; failure to attend and to have read the laboratory protocol in advance will place an undue burden on your lab partner. Each unexcused absence from lab will result in a reduction of one full letter grade (10 points) from your overall laboratory grade.If you know in advance that you will be absent (e.g., for sports), notify me as soon as possible or at least two weeks before the missed class, and we will try to schedule a make-up day. Make-up labs will be done at the discretion of the instructor and will most likely occur during one of the other sections. You are responsible for the material covered in class, and any assignments due that day must be turned in prior to the missed class. If you are ill, please notify me (e.g., via email) within 48 hours of the missed lab to set up an appointment to discuss the missed class (make-up labs may only be possible in a different lab section) and to establish deadlines for any missed assignments. If you do not contact me, then any missed assignment is due when you return to class. The absence will be excused only if a doctor’s note is provided. Please make an effort to arrive on time as instructions will be given at the beginning of each lab period and Pre-labs are due before the start of the introduction. Two unexcused "lates to lab" by 30 minutes or more will count as an unexcused absence. Academic Accommodations: Should you require academic accommodations, you must file a request with the Office of Educational Affairs (BC 114, extension 3327). It is your responsibility to self-identify with the Office of Educational?Affairs and to provide?me with the appropriate documentation from that office at least one week prior to any request for specific course accommodations.? We will set up a meeting to discuss the accommodations. There are no retroactive accommodations. Academic Integrity: Students are expected to strictly adhere to Drew University College of Liberal Arts Standards of Academic Integrity, see . You may discuss your assignment with others, but written assignments must be your own words and represent your understanding of the material. To avoid “accidental plagiarism” do not read someone else's written assignments (from past or current students) nor forget to properly cite your sources, including the lab protocol. Academic Integrity violations will be referred directly to the Dean.The following section of the Drew policy is repeated here as a reminder of academic integrity:“Unless a paper or assignment is collaboratively authored (and acknowledged as such), the presentation of the ideas, the interpretation of the data, and the organization of sentences and paragraphs should be original and should differ significantly from those in the papers or assignments of others who have collaborated on the research.”Lab Schedule: This is a general guideline, subject to change as needed. Corresponding assignment is indicated; parenthesis show the lab session in which the assignment is due. Assignment due dates are also included in the calendar; you are responsible for keeping track of deadlines. Assignments are accepted up to one week late, but 12/8 reading day is the last day for a late worksheet.LabExperimental TopicCorresponding Assignment1Nucleic Acid Isolation from Fruit2Human Genetic Variation: PCR3Human Genetic Variation: Digestion and AnalysisGenetic Variation lab report (4)4Plasmid Identification I: Isolation and Restriction Digest5Plasmid Identification II: Transformation and Agarose Gel Analysis6Plasmid Identification III: Reporter ExpressionPlasmid Identification lab report (7)7DNA Damage Sensitivity I 8DNA Damage Sensitivity II: Assessment DNA Damage lab report (9)9Introduction to Cell Culture10Mitosis I: Cell Immunostaining; Initiation of Western Blot11Mitosis II: Fluorescent MicroscopyImmuno worksheet (13)12Western Blot CompletionImmuno worksheet (13)13BioinformaticsBioinformatics worksheet in lab~ August/September 2010 ~SunMonTueWedThuFriSat30 Lab 131Lab 11 Lab 12 Lab 13 Lab 14 5 6 Labor Day: no classes7 Lab 2 8 Lab 29 Lab 210 Lab 211 12 13 Lab 214 Lab 315 Lab 316 Lab 317 Lab 318 19 20 Lab 321 Lab 4Genetic Variation lab report due22 Lab 4Genetic Variation lab report due23 Lab 4Genetic Variation lab report due24 Lab 4Genetic Variation lab report due25 26 27 Lab 4Genetic Variation lab report due28 Lab 529 Lab 530 Lab 5Notes:~ October 2010 ~SunMonTueWedThuFriSat1 Lab 52 3 4 Lab 55 Lab 66 Lab 67 Lab 68 Lab 69 10 11 Lab 612 Lab 7Plasmid Identification lab report due13 Lab 7Plasmid Identification lab report due14 Lab 7Plasmid Identification lab report due15 Lab 7Plasmid Identification lab report due16 17 18 Reading Day, no classes19 Reading Day, no classes20 MONDAYLab 7Plasmid Identification lab report due21 Lab 822 Lab 823 24 25 Lab 826 Lab 827 Lab 828 Lab 9DNA Damage lab report due29 Lab 9DNA Damage lab report due30 31 Notes:~ November 2010 ~SunMonTueWedThuFriSat1 Lab 9DNA Damage lab report dueStart of registration for Spring 2010 semester2 Lab 9DNA Damage lab report due3 Lab 9DNA Damage lab report due4 Lab 10 5 Lab 10 Last day to drop semester long classes with a “W”6 7 8 Lab 10 9 Lab 10 10 Lab 10 11 Lab 1112 Lab 1113 14 15 Lab 1116 Lab 1117 Lab 1118 Lab 12 19 Lab 12 20 21 22 Lab 12 23 Lab 1224 Thanksgiving recess begins, no classes25 Thanksgiving recess, no classes26 Thanksgiving recess, no classes27 28 29 Lab 13Immuno worksheet due30 Lab 13Immuno worksheet dueNotes:~ December 2010 ~SunMonTueWedThuFriSat1 Lab 12 2 Lab 13 Immuno worksheet due3 Lab 13 Immuno worksheet due4 5 6 WEDNESDAYLab 13 Immuno worksheet due7 Reading day, no classes8 Reading day, no classes9 Final exams beginGOOD LUCK!!10 11 12 13 14 15 Last day of finals16 17 18 Calendars created with WinCalendar ................
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