Lab #5 Cell Respiration (Lab Bench Activity #5)
IB1 HL 2018: Virtual Respirometry Lab
Click on the link above and answer the following questions in your lab notebook. Please write the section headings along with making sure you use full sentences in your responses.
Introduction and Key Concepts
1. What will you observe in this investigation?
2. Write the equation for cellular respiration.
3. What are some processes in plants that require respiration?
Design of the Experiment
4. What are the three ways in which you can measure the rate of cellular respiration?
5. What method will be used in this demonstration?
6. Sketch a respirometer and label its important features. What does it measure?
7. As the organism inside the respirometer consumes oxygen, what happens to the water?
8. What happens to the CO2 that the organism produces?
9. Experimental Setup: view the graphic, then create this data table in your notebook and fill it in.
| |Vial 1 |Vial 2 |Vial 3 |Vial 4 |Vial 5 |Vial 6 |
|Contents | | | | | | |
|Temperature | | | | | | |
10. How do you ensure that each vial has an equal volume?
11. What is the purpose of the vial with only glass beads?
Analyzing the Results
1. What is the equation to determine the rate of respiration?
2. What is x? What is y?
3. Read the respirometers and determine the rate of respiration. Show your calculations in your lab notebook.
4. Answer the four self-quiz questions in your lab notebook.
5. Answer the following questions relating to the data obtained from germinating corn seeds.
[pic]
a) Describe the relationship between temperature and consumption of oxygen.
b) Calculate the rate of oxygen consumption for germinating corn at 12 degrees. (Show calculations)
c) Based on the graph, would you conclude that non germinating seeds respire?
11. A cricket is placed in a respirometer and the amount of O2 consumed (in ml) is taken at three temperatures. Graph the data collected from the following table.
|Time (min) |Temperature |
| |10(C |18(C |25(C |
|0 |0.0 |0.0 |0.0 |
|5 |0.25 |0.6 |0.9 |
|10 |0.5 |0.9 |1.4 |
|15 |0.7 |1.2 |1.8 |
|20 |0.9 |1.6 |2.4 |
a) Determine the rate of respiration for each of the 3 temperatures. (Show calculations.)
Title: _____________________________________
b) Make a conclusion that answers the experimental question, “How does temperature affect the rate of respiration in crickets?” Use the data to justify your conclusion. Research why respiration rates vary at different temperatures and include this information in your conclusion.
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