The Effect of Natural Antibiotics on Homegrown Bacteria



The Effect of Natural Antibiotics on Homegrown BacteriaBy: Cassie Lavin2/26/2013Table of ContentsPage #(s) Content1……………………………………………………………………………………….…Title Page2………………………………………………………………………………….Table of Contents3-7……………………………………………………………………………….Literature Review8………………………………………………………………………………………Introduction8-9………………………………………………………..……………Experimental Design Chart9…………………………………………………………………………………………..Materials10………………………………………………………………………………………...Procedure11…………………………………………………………………………Observations and Data12…………………………………………………………………………………..….Conclusion13…………………………………………………………………………………….BibliographyLiterature ReviewsLiterature Review #1"Breeding Bacteria & Growing Microorganisms - Fun Science Experiments for Kids." Science for Kids - Fun Experiments, Cool Facts, Online Games, Activities, Projects, Ideas, Technology. N.p., n.d. Web. 9 Apr. 2013. < Notes:How to prepare agar dish.Pictures of types of bacteria.Dispose dishes wrapped in newspaper.Warm environment.Immune system helps right bacteria.Justification in paragraph form (Connect to the real world, applications, implications, background ) On this website I learned mostly how to prepare the agar dish. It also taught me how to safely dispose the dishes with newspaper. This was important because it’s part of your health, and if you are exposed to too much bacteria, you could get sick. One more thing it taught me was that your immune system helps fight off harmful bacteria to keep you healthy.Literature Review #2"The 5 best natural antibiotics and anti-virals that destroy superbugs and just about everything else." Natural health news. N.p., n.d. Web. 9 Apr. 2013. < Notes:GarlicEchinacea Colloidal SilverPau D’ArcoManuka HoneyMost are used in medicines and antibioticsJustification in paragraph form (Connect to the real world, applications, implications, background ) I learned a lot about the antibiotic portion of the project on this article. I learned that Garlic, Echinacea, Colloidal Silver, Pau D’Arco, and Manuka Honey are the top 5 natural antibiotics you can find in the world. I also learned that these are all used mostly in medicines and/or antibiotics used to fight off sickness and bacteria.Literature Review #3"Nutrient Agar Powder Preparation and Use- Science Fair Project Ideas - Tips." Science Stuff :: Educational Science Supplies, Toys, Games and Kits. N.p., n.d. Web. 9 Apr. 2013. < notes:Directions on making agar mixtureStack agar plates upside downDo not freezeRefrigerate Keep sterile dishes closed until inoculationGave me the recipeAgar extracted from red seaweedContains beef extracts Justification in paragraph form (Connect to the real world, applications, implications, background ) In this document I understood how to create the agar mixture used to grow the bacteria. It also gave a lot of key notes along the sides, which included safety notes. Some of those notes are listed above in the bulleted notes. Literature Review #4"Bacteria Science Project Guide with Video." Home Science Tools | Microscopes, Chemistry Supplies, Biology Supplies. N.p., n.d. Web. 7 Nov. 2012. < Notes:First website visitedNeed Petri dishes, agar, and sterile swabsOne set of proceduresVideo tutorialBacillus stearothermophilus, Branhamella catarhallis, and Bacillus coagulans are complex bacteria that require different nutrients, like tryptic soy agarTeaches how to kill the bacteriaJustification in paragraph form (Connect to the real world, applications, implications, background) This was the first website I visited. It was a very helpful one to look at for ideas on how to do the project and what it should look like. It gave me a general idea of the entire project. It gave me a video tutorial, a set of procedures, a set of directions on how to dispose the Petri dishes, and extra information on bacteria.Literature Review #5Wolfe, Nathan. "Microbes: Small, Small World." National Geographic Jan. 2013: n. pag. National Geographic. Web. 16 Nov. 2012.Bulleted Notes:When we inhale, our nostrils capture millions of invisible particles: dust, pollen, sea spray, volcanic ash, plant spores.They’re just passing through. Your nasal passages also host a rich and complicated population of full-time residents. Three genera—Corynebacterium, Propionibacterium, and Staphylococcus—account for most of the bacteria in your nostrils.Shows the different bacteria that can be found in different openings of the body.Justification in paragraph form (Connect to the real world, applications, implications, background) This was the second source I visited. It was a National Geographic article that I found online that explained what kinds of bacteria lived inside your body. This was helpful because it named what kind of bacteria was where, and it helped to understand what was dangerous, safe, etc. This was filled with great information that was very helpful. Introduction This experiment is important to everyone’s knowledge on health. Bacteria are everywhere. It’s in the air you breathe, the food you eat, and anything you touch. But nobody seems to understand that there are natural antibiotics that kill these bacteria. I thought that it would be interesting to try it out myself, so I chose to conduct this experiment. The purpose of this experiment is to determine the effects of natural antibiotics on home grown bacteria. This experiment will mock the bacteria that grow in the real world, and how these natural substances help fight off these harmful organisms. I hypothesize that if I combine the natural antibiotics with the home grown bacteria, the antibiotics will over rule. I believe so, because if it works in the real world, it should work in this experiment as well. This experiment is a scenario of real life events, so my hypothesis should be correct.Experimental Design Chart Title: The Effect of Natural Antibiotics on Homegrown Bacteria.Hypothesis: I hypothesize that if I combine natural antibiotics with homegrown bacteria, the antibiotics will dominate.DV: The dependants in this experiment are the bacteria dishes. This was measured when I first poured the Petri dishes; they all have the same amount in them. IV: The independent in this experiment is the bacteria. The bacteria are purposefully being grown and killed, to test what people say about natural antibiotics. Constants: A constant in this experiment are the natural antibiotics. These will be measured by the ? of a teaspoon. Another constant is the temperature in the room. This is measured by the heating lamp. MaterialsPetri dishesPowdered agarWater Sterile cotton swabsGarlic1/4 teaspoon measuring spoonHeat lamp StovePanHeat resistant gloveSterile glass stir rodProcedurePart 1:Measure agar and distilled water into a pan.Put pan over flame, and boil mixture for 1 minute.While boiling, stir with a sterile glass stir rod.After mixture has boiled for one minute, set aside and let stand a few minutes.Pour enough melted agar into each Petri dish to cover the bottom; about 1/8” to 1/4” deep.Replace lids immediately. Agar medium will settle like stiff gelatin at room temperature. When the agar has settled completely, swab areas of collection with sterile swab, and gently glide the surface of the swab over the agar mixture.Cover with lid immediately after inoculation. Wait a few days, and you should see results.Part 2:Now that your bacteria are grown, it’s time to kill it.Measure garlic with 1/4 teaspoon spoon. Sprinkle garlic over Petri dish.Wait a couple more days, and the bacteria should be killed. Observations and DataGraph 1This graph shows the overall growth and effect of both the bacteria and garlic. The garlic didn’t end up killing the bacteria, so that’s why it’s flat at the end. However, I summed up the information from the entire project and put it into this graph. Table 1Day102030405060ProgressScale 1-3082228282725This table shows the progress throughout the entire experiment. From when the bacteria was growing, to when I attempted to kill it with the garlic. As it shows, the bacteria grew at an accelerated rate, to a constant rate, then it decreased a very little bit.ConclusionThe purpose of the experiment was to figure out if the natural antibiotics would kill the homegrown bacteria that I grew. It was not only for this reason, but to learn more about my surroundings and things that happen unnoticed. It was a huge surprise whenever I found out that the garlic did not kill the bacteria. The first part of the experiment was a success (growing the bacteria). However, the second part wasn’t. After about a month and a half, the bacteria were fully developed and ready to begin the second part of the experiment. I sprinkled shredded garlic on the Petri dishes, and waited a few days. There was barely any change in the growth of the bacteria. My hypothesis did not support any of my data findings, because I guessed that the garlic would kill the bacteria. I have no idea why the garlic didn’t kill the bacteria, but I believe there are a few things I could’ve improved that might have improved the results of the experiment. I believe that if I would’ve researched into how to apply the garlic a little bit more, it would’ve improved the results. Other than that, the project was successful.If I were to give advice to other people about what else to improve on, it would be to make the temperature of the incubator/wherever you store your dishes higher than room temperature. If you store your dishes in a warmer environment, the bacteria will produce faster, which will make for an easier experiment. I’d also tell them a different way to apply the garlic, since my method wasn’t successful.Bibliography"Breeding Bacteria & Growing Microorganisms - Fun Science Experiments for Kids." Science for Kids - Fun Experiments, Cool Facts, Online Games, Activities, Projects, Ideas, Technology. N.p., n.d. Web. 9 Apr. 2013. <;"The 5 best natural antibiotics and anti-virals that destroy superbugs and just about everything else." Natural health news. N.p., n.d. Web. 9 Apr. 2013. <;"Nutrient Agar Powder Preparation and Use- Science Fair Project Ideas - Tips." Science Stuff :: Educational Science Supplies, Toys, Games and Kits. N.p., n.d. Web. 9 Apr. 2013. <;"Bacteria Science Project Guide with Video." Home Science Tools | Microscopes, Chemistry Supplies, Biology Supplies. N.p., n.d. Web. 7 Nov. 2012. <, Nathan. "Microbes: Small, Small World." National Geographic Jan. 2013: n. pag. National Geographic. Web. 16 Nov. 2012. ................
................

In order to avoid copyright disputes, this page is only a partial summary.

Google Online Preview   Download