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Cells on coverslips were fixed with 4% paraformaldehyde and permeabilized for 30 min at 37°C with 0.1% Triton X-100. Non-specific protein binding was blocked with 1% goat serum and cells were incubated with anti-RUNX2 primary antibody (1:100; NBP1-89104, Novus Biologicals) overnight at 4°C. ................
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