Microbiology College Course Laboratory Review Assignment



In-lab Review for Lab Practical

Differential Stains and Specialized Media

Name: __________________________________________________

1. Gram Stain: Prepare a bacterial smear using the two controls and unknown provided. Do a Gram stain. Is the unknown Gram + or Gram -? Draw and color label your controls and unknown to support your answer. Unknown # _______.

Color:______, + or -? Color: _______, + or -? Color:______, + or -?

2. Acid-fast Stain: There are scopes set up with the acid fast stain controls and unknown. Is your unknown AF (+) or NAF (-)? Draw and color label your controls and unknown to support your answer.

Color:______, + or -? Color: _______, + or -? Color:______, + or -?

3. Endospore Stain: There are scopes set up with the endospore stain controls and unknown. Is your unknown an endospore producer (+) or not (-)? Draw and color label your controls and unknown to support your answer.

Color:______, + or -? Color: _______, + or -? Color:______, + or -?

4. Describe all of the information provided by the specialized medium #1.

5. Describe all of the information provided by the specialized medium #2.

See the Virtual Microbiology Classroom of the Science Prof Online website for more microbiology course material.

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NOTE: These questions are unrelated to each other. The unknown for each is not necessarily the same. Answer each question independently.

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