WHONET Tutorial



WHONET

Laboratory Configuration

WHO Collaborating Centre for

Surveillance of Antimicrobial Resistance

Boston, Massachusetts

June 2006

WHONET Tutorial – Laboratory Configuration

This tutorial includes the following sections.

Part 1. Describing your laboratory

Part 2. Selecting your antibiotics

Part 3. Configuring your antibiotics

Part 4. Patient locations

Part 5. Data fields

Part 6. Isolate alerts

Part 7. Finishing laboratory configuration

The purpose of laboratory configuration is to describe to WHONET details about your institution and your laboratory test practices. This tutorial describes how to create a “new laboratory” from the very beginning.

Note: If you will be using BacLink, there is a short-cut to accomplish most of the following steps. See the BacLink tutorials for more information. The short-cut feature is called “Create a laboratory from a data file”, and can be found under the WHONET “File” menu option.

Part 1. Describing your laboratory

Double-click on the WHONET icon on your desktop to begin WHONET. You will see a list of laboratory configurations currently defined on your computer. Click on “New Laboratory” to begin.

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Figure 1. List of WHONET laboratory configurations. Select “New laboratory”.

In this tutorial, you will create an institution in the special “country” World Health Organization called “WHONET Tutorial Hospital”. So for country, select “World Health Organization”, and for the laboratory name, type “WHONET Tutorial Hospital”. For the laboratory code, put “WTH”. Your screen should look like the below.

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Figure 2. WHONET laboratory configuration screen. Enter the country, name, and code of your laboratory.

Part 2. Selecting your antibiotics

The only part of laboratory configuration which is required is indicating which antimicrobials you are testing in your laboratory. To do this, click on “Antibiotics” to get the following screen. You will see a long list of antibiotics to your left – this is the WHONET list. On the right is the list of antibiotic tests used in your laboratory. At the beginning this list is empty.

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To indicate the tests that you are using, you should indicate three things: 1. the reference guidelines (for example CLSI, SFM, DIN, etc.); 2. the test method (disk, MIC, or Etest); and 3. the name of the antibiotic and, for disk diffusion testing, the disk potency.

In this tutorial, indicate that the method is CLSI, and you will choose a few drugs tested by disk diffusion and a few tested by Etest. To select an antibiotic, double-click on the antibiotic to move it to the right side of the screen or single-click on the antibiotic and hit the “–>” button.

Find the following antibiotics, and move them to the right side of the screen.

Disk diffusion, ampicillin 10ug

Disk diffusion, cefoxitin 30ug

Disk diffusion, ceftriaxone 30ug

Disk diffusion, ciprofloxacin 5ug

Disk diffusion, erythromycin 15ug

Disk diffusion, gentamicin 10ug

Disk diffusion, imipenem, 10ug

Disk diffusion, penicillin G 10units

Disk diffusion, trimethoprim/sulfamethoxazole 1.25ug/23.75ug

Disk diffusion, vancomycin, 30ug

WHONET assigns a code to each antibiotic test, for example AMP_ND10 indicates a test of ampicillin (“AMP”) by CLSI (“N”, formerly NCCLS) methods by disk diffusion (“D”) with a 10ug disk (“10”).

Now enter a few drugs tested by Etest. Click on the option labeled “Etest” and select the following drugs. Since the disk potency is not relevant for Etests, it does not matter which ”ceftriaxone” you select from the WHONET list.

Etest, ceftiaxone

Etest, penicillin G

Etest, vancomycin

The corresponding test code for the ceftriaxone Etest done by CLSI (formerly NCCLS) methods would be CRO_NE. After making these selections, you should have the following.

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Figure 3. Antibiotic configuration screen. Select from the list of antibiotics shown to the left of the screen. The antibiotic tests used in your laboratory appear to the right of the screen.

If you would like to change the sequence of antibiotics, you can use the “Move up” and “Move down” options. Or you can use the left arrow button “ ................
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