Example of Oxygen Depletion Calculations - Monash University
Example of Oxygen Depletion Calculations
Nitrogen is the main component of air and is present at approximately 78% by volume, the other major components being oxygen, approximately 21% and argon, approximately 1%. Depletion of the concentration of oxygen can have an effect on life. An atmosphere containing less than 18% oxygen is potentially hazardous and entry into areas with atmospheres less than 20% oxygen is not recommended. Asphyxiation due to low oxygen concentrations is often rapid and with no prior warning.
If you are using liquid nitrogen or non-hazardous compressed gases (DG class 2.2) in cylinders, you should be aware of the potential for oxygen depletion in the work or storage area. This can be calculated, typically assuming that all power to ventilation systems has failed (eg in a fire or a blackout) and a sudden spillage of liquid nitrogen or release of gas.
Resulting Oxygen Concentration (%):
0.2095 x (Vr - Vg)
%O2 = 100 X
Vr
Vr = room volume (m3)
Vg = maximum gas release (m3)
A G size cylinder of an inert gas contains approximately 6-8 m3 of gas. For liquefied gases, the expansion ratios are ? N2 678, Ar 824, He739, ie 1 L of liquid nitrogen produces 678 L of or 0.678 m3 of gas.
A typical laboratory in the School of Chemistry is approximately 250 m3 (eg level 1, 109-125 and 152173) is ca 8.75 x 7.5 x 3.0 m (ie Vr = 200 m3) or in GCF (eg level 3, 302-306 and 310-315) is ca 6.25 x 12.50 x 3.5 m (ie Vr = 270 m3)
A 25L dewar of liquid nitrogen would produce 25 x 0.678 = 16.95 m3 of gas (Vg).
Therefore:
%O2 = 0.2095 x (250 ? 16.95)/250 = 19.5%
If the calculation suggests a resultant oxygen content below 18% then further controls should be considered, such as a) smaller dewar capacities b) low oxygen alarm c) reticulated gas supply.
References:
AS: 1894-1997 The Storage and Handling of Non-flammable Cryogenic and Refrigerated Liquids BOC Guidelines for Gas Cylinder Safety
Oxygen Depletion Document v1 School of Chemistry Date of first issue: June 2018
Responsible Officer: Safety Officer Page 1 of 1 Date of next review: N/A
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