Course Title:
Course Title: Histopathology
Course Number: MeLS 432
Credit Hours: 3
Lecture: 2 hours/week
Laboratory: 3 hours/week
Prerequisite: None
Instructor:
Course Objective:
At the end of the course the students will able to describe the basic concepts of histopathology, and apply histopathological techniques in the diagnosis of tissue abnormality
Supporting objectives
Upon completion of the course, the student will be able to:
1. Define Histopathology.
2. Discuss pathologic tissue changes
3. Discuss purposes of histopathological examinations
4. Apply laboratory safety as it is related to all aspects of the Histology laboratory.
5. Process surgical and autopsy tissue specimens.
6. Correctly and safely operate a rotary microtome to section paraffin processed blocks of tissue.
7. Stain smears and sections with routine and special staining procedures.
8. Critically evaluate the quality of the sectioning and staining of prepared slides.
9. Use knowledge of applied chemistry to solve technical problems as they are encountered in histology.
10. Perform corrective action at any stage of histopathological techniques that are inadequate.
11. Describe the different types of specimen in histopathology (pathology) laboratory
12. List and practice methods of preservation of the various specimen
13. Carry out pathological tests using the various staining techniques
14. Prepare biological material for microscopic evaluation.
15. Perform cell concentration and fixation techniques,
16. Recognize and maintain laboratory quality control in histopathology laboratory
Course description:
This is a 3 credit hour course designed to equip BSC degree students of Medical Laboratory Technology with basic Knowledge of pathology and diagnostic techniques in pathology. The course give more emphasis on histopathological techniques, fixation and fixative, tissue processing, tissue sectioning, staining and Immunocytochemistry of histopathological diagnosis.
Course outline:
Chapter One
1. Introduction to pathology (8 HOURS)
1. Etiology, morphology, pathogenesis and manifestation
2. Reversible and irreversible changes
3. Inflammation
4. Neoplasia
Chapter two
2. Fixation and fixatives (3hrs)
1. Introduction
2. purpose of fixation
3. Surgical specimen collection and handling
4. classification of fixative
5. additives to fixatives
6. factors involved in fixation
7. fixative to specific substances
8. Decalcification
CHAPTER Three
3. Tissue processing (3hrs)
1. Introduction
2. Dehydration
3. Dehydration fluids
4. clearing
5. Impregnation and embedding
6. Factors influencing the rate of tissue processing
Chapter Four
4. Tissue sectioning (2hrs)
1. Introduction
2. Microtomy
3. Type of microtome knife
4. frozen section
Chapter Five
5. Principles of staining (6 hours)
1. Type of stain
2. Affinity of stain
3. factors determining sensitivity of stains
4. Hematoxyline staining
5. Common stains in Histological techniques
6. Mounting
Chapter Six
6. Special staining methods (4hours)
1. Connective tissue staining
2. Protein, nucleic acid and amyloid
3. Carbohydrates and lipids
4. Pigments, Minerals and Bone
5. Neuroendocrine and Neuropathology techniques
6. Microorganisms
Chapter Seven
7. Immunocyto/histochemistry (2hrs)
1. Histochemistry
2. Enzyme histochemistry
3. Immunostaining
4. Immunoflourescence
5. Autoradiography
Chapter Eight
8. Cytology and museum techniques(4hours)
1. Introduction to Cytology
2. Pathology museum techniques
Learning materials:
Lecture notes, chalk and board, white board, overhead projector and transparency, laptop and LCD
References:
1. Histology. Alan Stevens, James Lowe. 1992
2. Cellular and molecular immunology. Abul K. Abbas, Adrew H. Lichtman. 5th ed. 2003
3. John D Bancroft. Marilyn Gamble. Theory and practice of histopathological techniques. 5th edition.
4. Basic pathology. 6th ed. 1997
5. Robbins. Pathologic basis of disease 6th edition. 1999
6. Berhanu S. Jemal Y. Histopathology lecture note Series, Haramaya university, 2007.
7. Luis carlos Junquwira. Basic histology, 9th edition, 1998.
Teaching Methods:
✓ Lecture
✓ Demonstration
✓ Presentation and group discussion
✓ Laboratory practice
✓ Audiovisual
Assessment Method:
➢ Assignment & Quiz (10%)
➢ Laboratory report (10%)
➢ Practical examination (20%)
➢ Mid examination (20%)
➢ Final examination (40%)
Time Allocation: 80 contact hours (32 lectures + 48 labs)
Grading: Fixed scale
Type of Examination:
Theory:
( Multiple choice questions, Short answers, Essays (minimum)
➢ Matching, Completion and True or false (Optional)
Practical:
➢ Display (spotter)
➢ Examination of specimen
➢ Demonstration of Lab procedures
➢ Identification
➢ Oral examination
Laboratory Practice
The students should have to practice the following till the end of the course
➢ Surgical and autopsy tissue specimens processing
➢ Microtome Sectioning of paraffin embedded blocks of tissue
➢ Staining of smears and sections with routine and special staining procedures.
➢ Examination of stained pathological smears
➢ Preservation of the various pathological specimen
➢ Cell concentration and fixation techniques,
................
................
In order to avoid copyright disputes, this page is only a partial summary.
To fulfill the demand for quickly locating and searching documents.
It is intelligent file search solution for home and business.
Related searches
- free financial management course online
- personal financial management course online
- financial management course online
- early childhood education course online
- personal finance management course bankruptcy
- personal financial management course navy
- basic airborne course packing list
- financial management course syllabus
- excel training course pdf
- financial management course outline
- writing course middle school
- bankruptcy financial management course free