FIVEphoton Biochemicals Gene Synthesis and Subcloning ...
FIVEphoton Biochemicals Gene Synthesis and Subcloning Quote Request
Instructions:
Please fill in the following information, save the file on your computer, and email as an attachment to genesynthesis@. Space allocation to your answer is flexible. We will return your quote and a description of the project shortly to your email address.
__________________________________________________________________________________________
Last Name: ________________________________________________
First Name: _______________________________________________
Institution/Company: _______________________________________
Address: _____________________________________________
________ _____________________________________
______________________________________________
Telephone Number: _____________________________________
Email Address: __________________________________________
Provide a brief project summary of your project. For example: " I will provide the expression vector pcDNA3-HERG to FIVEphoton Biochemicals and request subcloning into position 354 of the gene sequence provided below. Please generate a codon optimized gene for human cells, with the reading frame staring at position 3."
Enter project summary:
Paste requested gene sequence here (In FASTA format, 5’ to 3’):
Do you request codon optimization: (yes or no): _____________
If requesting codon optimization, please provide the following information:
a. List first three nucleotides at the 5’ end that designate the reading frame:
b. Species of cells gene will be expressed:
Do you request insertion of the synthesized gene in the standard vector pGH. We will use a blunt end cloning strategy and preserve your entire sequence:
Yes or no: ____________
Do you request custom subcloning into an expression vector or insertion into a gene (customer will ship to FIVEphoton Biochemicals 1μg of lyophilized (speed vac’d) plasmid with inserted gene.
Yes or no: ___________
If requesting custom subcloning, please provide the following information:
1. Name of expression vector and inserted gene.
2. Accession number of inserted gene.
3. Please insert the FASTA sequence of the parent gene.
4. Indicate the cloning sites for subcloning of the synthesized gene.
5. Provide the final peptide sequence that includes the parent gene and the synthesized
gene.
Include any special requests:
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